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Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae

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Abstract

The role of acidic SKn dehydrins in stress tolerance of important crop and model species of the Solanaceae remains unknown. We have previously shown that the acidic SK3 dehydrin DHN24 from Solanum sogarandinum is constitutively expressed and its expression is associated with cold acclimation. Here we found that DHN24 is specifically localized to phloem cells of vegetative organs of non-acclimated plants. More precise localization of DHN24 revealed that it is primarily found in sieve elements (SEs) and companion cells (CCs) of roots and stems. In cold-acclimated plants, DHN24 is mainly present in all cell types of the phloem. Dhn24 transcripts are also predominantly localized to phloem cells of cold-acclimated stems. Immunoelectron microscopy localized DHN24 to the cytosol and close to organelle membranes of phloem cells, the lumen with phloem protein filaments, parietal cytoplasm of SEs and the nucleoplasm of some nuclei. Cell fractionation experiments revealed that DHN24 was detected in the cytosolic, nuclear and microsomal fractions. We also determined whether homologous members of the acidic subclass dehydrins from Capsicum annuum and Lycopersicon chilense share the characteristics of DHN24. We showed that they are also constitutively expressed, but their protein level is upregulated preferentially by drought stress. Immunofluorescent localization revealed that they are detected in SEs and CCs of unstressed plants and throughout the phloem in drought-stressed plants. These results suggest that one of the primary roles of DHN24 and its homologs may be the protection of the phloem region from adverse effects of abiotic stresses.

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Abbreviations

CC:

Companion cell

SE:

Sieve element

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Acknowledgments

Prof. Zbigniew Miszalski (IPP, PAS, Cracow) is acknowledged for comments on the manuscript. Malgorzata Wasilewska-Gomulka is acknowledged for ultramicrotome work. This work was supported in part by the Ministry of Science and Higher Education, Grant No. PBZ/MNiSW-2/3/2006.

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The authors declare that they have no conflict of interest.

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  1. Bartosz Mieczyslaw Szabala

    Present address: Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences, SGGW, Nowoursynowska St 159, 02-776, Warsaw, Poland

Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479, Poznan, Poland

    Bartosz Mieczyslaw Szabala & Tadeusz Rorat

  2. Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, SGGW, Nowoursynowska St 159, 02-776, Warsaw, Poland

    Sylwia Fudali

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  1. Bartosz Mieczyslaw Szabala
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Correspondence to Bartosz Mieczyslaw Szabala.

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Szabala, B.M., Fudali, S. & Rorat, T. Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta 239, 847–863 (2014). https://doi.org/10.1007/s00425-013-2018-6

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