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Quantitative genetic analysis and mapping of leaf angle in durum wheat

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Abstract

The leaf erectness profile has been used to optimize plant architecture since erect leaves can enhance photosynthesis and dry matter production by greater sunlight capture. Brassinosteroid is a recent class of phytohormones that has been related to a more erect profile. There are no reports in the literature of the genetic variability of leaf angle in doubled haploid durum wheat populations; most studies on leaf angle have focused on the inheritance. Our aim was to study the genetic variation in flag and penultimate leaf angle in a durum wheat doubled haploid mapping population, identifying and mapping quantitative trait loci influencing leaf angle. An F1-derived doubled haploid population of 89 lines from the cross Strongfield/Blackbird was used to construct a genetic map using 423 molecular marker loci. Two greenhouse experiments and one field test were conducted using an alpha lattice in a randomized complete block design with three replicates. The leaf angle was measured on flag and penultimate leaf with a protractor at three different growth stages. The results indicated poor to moderate correlations between the position of the leaf angle and the growth stage. Transgressive segregation beyond Strongfield and Blackbird of leaf angle was observed for all environments. Putative trait loci were identified on chromosomes 2A, 2B, 3A, 3B, 4B, 5B and 7A. This work helps to understand the genetics of leaf angle in durum wheat.

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Abbreviations

BR(s):

Brassinosteroid(s) class of polyhydroxy plant steroid hormones first discovered in Brassica napus

DH:

Doubled haploid

QTL:

Quantitative trait loci

PCR:

Polymerase chain reaction

Taq :

Thermus aquaticus enzyme

LSMEANS:

Least square means

KW:

Kruskal–Wallis test

MQM:

Multiple QTL mapping

LOD:

Logarithm of the odds

PDIFF:

Requests that difference of the LS means is displayed

FL:

Flag leaf

2L:

Penultimate leaf

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Acknowledgments

This research was funded by AAFC’s ABIP as part of the Agriculture Biorefinery Innovation Network ABIN Canada and the Ministry of Economy and Competitiveness of Spain. The authors acknowledge Errol Stuart for his greenhouse support, as well as, the technical assistance of Alison Banman, Theresa Colenutt, Brad Meyer, Cam Barlow and Chelsea Erickson.

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada (AAFC), Semiarid Prairie Agriculture Research Centre, Swift Current, SK, S9H 3X2, Canada

    Julio Isidro, Ron Knox, Fran Clarke, Asheesh Singh & Ron DePauw

  2. Department of Plant Sciences, University of Saskatchewan, Saskatoon, S7N 5A2, Canada

    John Clarke

  3. Vineland Research and Innovation Centre, Vineland, ON, L0R 2E0, Canada

    Daryl Somers

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  1. Julio Isidro
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  2. Ron Knox
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Correspondence to Julio Isidro or Ron Knox.

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Isidro, J., Knox, R., Clarke, F. et al. Quantitative genetic analysis and mapping of leaf angle in durum wheat. Planta 236, 1713–1723 (2012). https://doi.org/10.1007/s00425-012-1728-5

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