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Advances in understanding pectin methylesterase inhibitor in kiwi fruit: an immunological approach

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Abstract

In order to gain insight into the in situ properties and localisation of kiwi pectin methylesterase inhibitor (PMEI), a toolbox of monoclonal antibodies (MA) towards PMEI was developed. Out of a panel of MA generated towards kiwi PMEI, three MA, i.e. MA-KI9A8, MA-KI15C12 and MA-KI15G7, were selected. Thorough characterisation proved that these MA bind specifically to kiwi PMEI and kiwi PMEI in complex with plant PME and recognise a linear epitope on PMEI. Extract screening of green kiwi (Actinidia deliciosa) and gold kiwi (Actinidia chinensis) confirmed the potential use of these MA as probes to screen for PMEI in other sources. Tissue printing revealed the overall presence of PMEI in pericarp and columella of ripe kiwi fruit. Further analysis on the cellular level showed PMEI label concentrated in the middle lamella and in the cell-wall region near the plasmalemma. Intercellular spaces, however, were either completely filled or lined with label. In conclusion, the developed toolbox of antibodies towards PMEI can be used as probes to localise PMEI on different levels, which can be of relevance for plant physiologists as well as food technologists.

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Abbreviations

fPME:

Aspergillus aculeatus pectin methylesterase

MA:

Monoclonal antibody

PME:

Pectin methylesterase

PMEI:

Pectin methylesterase inhibitor

t1PME:

Tomato (Solanum Lycopersicon) pectin methylesterase

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Acknowledgments

This research has been carried out with financial support from the Research Council K.U.Leuven (IDO/05/011). S. Christiaens and S. Van Buggenhout are researchers funded by the Research Foundation - Flanders (FWO). The authors would like to acknowledge ProMeta, the Interfacultary Centre for Proteomics and Metabolomics for the MALDI-TOF-MS analysis. Sincere thanks goes to Prof. J. Hofkens for giving us access to Olympus Fluoview 1000 confocal microscope.

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Authors and Affiliations

  1. Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Department of Microbial and Molecular Systems (M2S), Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, Postbox 2457, 3001, Leuven, Belgium

    Evelien Vandevenne, Stefanie Christiaens, Sandy Van Buggenhout, Ruben P. Jolie, Margarita González-Vallinas, Thomas Duvetter, Marc E. Hendrickx & Ann Van Loey

  2. Laboratory for Pharmaceutical Biology, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, O&N II Herestraat 49, Postbox 824, 3000, Leuven, Belgium

    Paul J. Declerck & Ann Gils

Authors
  1. Evelien Vandevenne
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  2. Stefanie Christiaens
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  3. Sandy Van Buggenhout
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  4. Ruben P. Jolie
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  5. Margarita González-Vallinas
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  6. Thomas Duvetter
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  7. Paul J. Declerck
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  8. Marc E. Hendrickx
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  9. Ann Gils
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  10. Ann Van Loey
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Correspondence to Ann Van Loey.

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Vandevenne, E., Christiaens, S., Van Buggenhout, S. et al. Advances in understanding pectin methylesterase inhibitor in kiwi fruit: an immunological approach. Planta 233, 287–298 (2011). https://doi.org/10.1007/s00425-010-1307-6

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  • DOI: https://doi.org/10.1007/s00425-010-1307-6

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