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Expression of a novel NAC domain-containing transcription factor (CaNAC1) is preferentially associated with incompatible interactions between chili pepper and pathogens

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Abstract

We aim to isolate genes in chili pepper that are regulated during the hypersensitive response to infection by nonhost pathogens, with a view to elucidating the defense responses against pathogen attack. Among the 90 transcription factors initially characterized by reverse RNA gel blot analysis, a cDNA clone, CaNAC1 (Capsicum annuum NAC1) containing the plant-specific NAC domain motif was further characterized. Expression of the CaNAC1 gene was rapidly and specifically induced during incompatible interactions between pepper and bacterial or viral pathogens. Additionally, this gene was strongly induced by exogenously applied salicylic acid and ethephon, whereas methyl jasmonate only had a transient effect. A CaNAC1-smGFP (soluble modified green fluorescent protein) fusion protein localized to the nucleus following transfection into the epidermis of onion. Using the yeast system, we further disclose that the transcription activation domain of CaNAC1 is located in the C-terminal half of the protein. Our results collectively suggest that the plant-specific NAC domain protein, CaNAC1, may play a role in the regulation of defense responses in plants.

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Abbreviations

CaNAC1 :

Capsicum annuum NAC1

SA:

Salicylic acid

JA:

Jasmonic acid

Xag :

Xanthomonas axonopodis pv. glycines

Xcv :

X. campestris pv. vesicatoria

Pss 61:

Pseudomonas syringae pv. syringae 61

PMMV:

Pepper mild mottle virus

PVP:

Polvinlypyrrolidone

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Acknowledgements

The authors thank Dr. Chung Eunsook (KRIBB) and Mr. Ahn Joon-Woo (KRIBB) for excellent technical support. This research was supported by grants from the Plant Diversity Research Center (PDRC), and the Crop Functional Genomics Center (CFGC) of the twenty-first Century Frontier Research Program funded by MOST and, in part, by KRIBB basic research fund.

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Authors and Affiliations

  1. Principal Research Scientist Plant Genomics Lab, Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, 52, Eoeun-dong, Yuseong-gu, Daejon, 305-333, Republic of Korea

    Sang-Keun Oh, Sanghyeob Lee & Doil Choi

  2. Plant Pathology Lab, Chungnam National University, Daejon, 305-764, Republic of Korea

    Seung Hun Yu

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  1. Sang-Keun Oh
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  2. Sanghyeob Lee
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  3. Seung Hun Yu
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  4. Doil Choi
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Correspondence to Doil Choi.

Additional information

CaNAC1 GenBank accession number, AY714222

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Oh, SK., Lee, S., Yu, S.H. et al. Expression of a novel NAC domain-containing transcription factor (CaNAC1) is preferentially associated with incompatible interactions between chili pepper and pathogens. Planta 222, 876–887 (2005). https://doi.org/10.1007/s00425-005-0030-1

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