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Satellite cell ablation attenuates short-term fast-to-slow fibre type transformations in rat fast-twitch skeletal muscle

  • Muscle Physiology
  • Published:
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Abstract

The purpose of this time-course study was to determine whether satellite cell ablation within rat tibialis anterior (TA) muscles exposed to short-term chronic low-frequency stimulation (CLFS) would limit fast-to-slow fibre type transformations. Satellite cells of the left TA were ablated by exposure to γ-irradiation before 1, 2, 5 or 10 days of CLFS and 1 week later where required. Control groups received only CLFS or a sham operation. Continuous infusion of 5-bromo-2′-deoxyuridine revealed that CLFS first induced an increase in satellite cell proliferation at 1 day, up to a maximum at 10 days over control (mean ± SEM, 5.7 ± 0.7 and 20.4 ± 1.0 versus 1.5 ± 0.2 mm−2, respectively, P < 0.007) that was abolished by γ-irradiation. Myosin heavy chain mRNA, immunohistochemical and sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses revealed CLFS-induced fast-to-slow fibre type transformation began at 5 days and continued at 10 days; in those muscles that were also exposed to γ-irradiation, attenuation occurred within the fast fibre population, and the final fast-twitch to slow-twitch adaptation did not occur. These findings indicate satellite cells play active and obligatory roles early on in the time course during skeletal muscle fibre type adaptations to CLFS.

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Acknowledgements

This study was funded by research grants from the Natural Sciences and Engineering Council of Canada (NSERC; to C.T. Putman), the Alberta Heritage Foundation for Medical Research (AHFMR; to C.T. Putman), the Alberta Agricultural Research Institute (to W.T. Dixon and G.R. Foxcroft) and the Canadian Institute for Health Research (to T. Gordon). K.J.B. Martins was supported by NSERC and AHFMR graduate scholarships. M. Gallo was supported by NSERC and Izaak Walton Killam Memorial scholarships. The authors thank Prof. S. Schiaffino (Padova, Italy) for generously providing monoclonal antibody clone BF-35 (purified IgG, not MHCIIx). The authors also thank M. Burgquest for technical assistance. T. Gordon is an AHFMR Senior Investigator, and C.T. Putman is an AHFMR Senior Scholar.

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Authors and Affiliations

  1. Exercise Biochemistry Laboratory, Faculty of Physical Education and Recreation, University of Alberta, Edmonton, AB, Canada, T6G 2H9

    Karen J. B. Martins, Yang Shu, R. Luke W. Harris, Maria Gallo & Charles T. Putman

  2. Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, AB, Canada, T6G 2P5

    Gordon K. Murdoch, Walter T. Dixon & George R. Foxcroft

  3. Division of Physical Medicine and Rehabilitation, University of Alberta, Edmonton, AB, Canada, T6G 2S2

    Tessa Gordon

  4. The Centre for Neuroscience, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada, T6G 2S2

    R. Luke W. Harris, Tessa Gordon & Charles T. Putman

  5. E-417 Van Vliet Centre, University of Alberta, Edmonton, Alberta, Canada, T6G 2H9

    Charles T. Putman

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  1. Karen J. B. Martins
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  2. Gordon K. Murdoch
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  9. Charles T. Putman
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Correspondence to Charles T. Putman.

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Martins, K.J.B., Murdoch, G.K., Shu, Y. et al. Satellite cell ablation attenuates short-term fast-to-slow fibre type transformations in rat fast-twitch skeletal muscle. Pflugers Arch - Eur J Physiol 458, 325–335 (2009). https://doi.org/10.1007/s00424-008-0625-z

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