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Role of anterior hypothalamic natriuretic peptide in lipopolysaccharide-induced fever in rats

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Abstract

We investigated whether within the preoptic area, natriuretic peptide (NP) acts as an endogenous antipyretic in rats made febrile by systemic administration of bacterial endotoxin (lipopolysaccharide, LPS). Intravenous (i.v.) injection of LPS (2 μg/kg) induced a triphasic fever. The third phase of this fever was (a) significantly enhanced by an intrapreoptic (i.p.o.) injection of the NP-receptor (A-type and B-type) antagonist HS-142-1(1 μg), and (b) significantly attenuated by an i.p.o. injection of atrial NP (ANP, 20 ng). When given i.v., LPS induced significant upregulation of the mRNA coding for C-type NP within the anterior hypothalamus, and tended to upregulate that for ANP. The anterior hypothalamic expression of interleukin-1β mRNA was significantly greater in rats injected i.v. with LPS than in saline-injected rats. These results suggest that NPs produced within the anterior hypothalamus after i.v. injection of LPS may act upon preoptic NP receptors to inhibit the LPS-induced fever, possibly through attenuation of the LPS-induced production of proinflammatory cytokines and/or the subsequent final fever mediator prostaglandin E2.

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Acknowledgments

We are grateful to Dr. Robert J. Timms for his critical reading of the English manuscript. We thank Kyowa Hakko Kirin Co. Ltd for the kind supply of HS-142-1. We would like to express our gratitude to Dr Makoto Taniguchi for his valuable suggestions. This work was partly supported by the Ministry of Education, Science and Culture with a Grant-in-Aid for Scientific Research (C19590225).

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  1. Division of Integrative Physiology, Department of Functional, Morphological and Regulatory Science, Tottori University Faculty of Medicine, Yonago, Tottori, 683, Japan

    Michio Miyoshi & Tatsuo Watanabe

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  1. Michio Miyoshi
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  2. Tatsuo Watanabe
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Correspondence to Tatsuo Watanabe.

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Miyoshi, M., Watanabe, T. Role of anterior hypothalamic natriuretic peptide in lipopolysaccharide-induced fever in rats. Eur J Appl Physiol 109, 49–57 (2010). https://doi.org/10.1007/s00421-009-1157-8

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