Abstract
Cell protrusive motility underlies cell fundamental biological processes such as cell growth, locomotion, and migration. Here I showed that selenium-binding protein (SBP) was exclusively located at the leading edges of rapidly growing protrusions in newly plated T98G glioma cells, and at the growing tips of the neurites in SH-SY5Y neuroblastoma cells. Double staining by anti-SBP antibody and deoxyribonuclease (DNase I) that labels monomeric G-actin or phalloidin that labels filamentous F-actin showed that the SBP-positive area was overstained by DNase I but, surprisingly, was not stained by phalloidin. When the cells were incubated with chemicals which block actin polymerization or activity of phosphatidylinositol 3-kinase, recruitment of SBP and G-actin at the cell margin was still observed, showing that their recruitment precedes actin polymerization. Taken together, I suggest that SBP may be involved in the initial sequential events in rapid cell outgrowth, such as determining direction of cell outgrowth and recruitment of actin monomer.
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Acknowledgements
I thank Dr. S. Sakoda for helpful advice and support. I also thank Dr. M. Shibata for anti-human SBP antibody and Dr. Y. Takai for helpful discussion.
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Miyaguchi, K. Localization of selenium-binding protein at the tips of rapidly extending protrusions. Histochem Cell Biol 121, 371–376 (2004). https://doi.org/10.1007/s00418-004-0623-y
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DOI: https://doi.org/10.1007/s00418-004-0623-y