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Revealing details of stays abroad by sequential stable isotope analyses along human hair strands

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Abstract

Multi-element stable isotope analyses of δ13C, δ15N, δ34S and δ2H values were performed along scalp hair strands to detect isotopic changes resulting from different stays abroad. One hair strand with a hair length of more than 50 cm originated from a German woman, who frequently made long-distance travels of 1 to 4 weeks. The second hair strand with a length of 15 cm was taken from a Japanese woman who went to Germany for a period of some months. Stable isotopic influences due to the stays abroad were clearly reflected in the 5-mm segments along the proximal part of the hair strand; whereas in the more distal parts, the isotopic influences were blurred. This can be regarded as the result of the highly variable intra-individual hair growth rate of single hairs of at least ± 30% compared to the mean growth rate. Consequently, the initial isotope signal obtained by short stays abroad became rapidly attenuated in the more distal parts of the hair strand. Furthermore, decreasing sulphur content associated with higher sulphur isotope values was observed with increasing hair length. The isotope shifts along the scalp hair strand, provoked by dietary changes at new locations, appeared at such points of hair length, which correspond well with the maximum growth rate of single hairs. Consequently, the exact date for any changes coming along with isotopic shifts may be calculated by best approach considering a hair growth value of 1.4 cm per 30 days, instead of the commonly used mean monthly hair growth rate of 1.1 cm. This may be important in forensics, if detailed information about a person’s recent lifetime should be figured out by segmental scalp hair analyses.

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References

  1. Lehn C, Rossmann A, Graw M (2015a) Provenancing of unidentified corpses by stable isotope techniques–presentation of case studies. Sci Justice 55(1):72–88

    PubMed  Google Scholar 

  2. Lehn C, Graw M (2016) Identifizierung einer skelettierten, Kofferleiche aus Berlin. Identification of a skeletonised suitcase corpse from Berlin, Germany. Rechtsmedizin 26(5):429–435

    Google Scholar 

  3. Nakamura K, Schoeller D, Winkler F, Schmidt HL (1982) Geographical variations in the carbon isotope composition of the diet and hair in contemporary man. Biol Mass Spectrom 9(9):390–394

    CAS  Google Scholar 

  4. Mützel E, Lehn C, Peschel O, Hölzl S, Roßmann A (2009) Assignment of unknown persons to their geographical origin by determination of stable isotopes in hair samples. Int J Legal Med 123(1):35–40

    Google Scholar 

  5. Valenzuela LO, Chesson LA, Bowen GJ, Cerling TE, Ehleringer JR (2012) Dietary heterogeneity among western industrialized countries reflected in the stable isotope ratios of human hair. PLoS One 7(3):e34234

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Valenzuela LO, Chesson LA, O’Grady SP, Cerling TE, Ehleringer JR (2011) Spatial distributions of carbon, nitrogen and sulfur isotope ratios in human hair across the Central United States. Rapid Commun Mass Spectrom 25(7):861–868

    CAS  PubMed  Google Scholar 

  7. Hülsemann F, Lehn C, Schneiders S, Jackson G, Hill S, Rossmann A, Scheid N, Dunn PJ, Flenker U, Schänzer W (2015) Global spatial distributions of nitrogen and carbon stable isotope ratios of modern human hair. Rapid Commun Mass Spectrom 29(22):2111–2121

    PubMed  Google Scholar 

  8. Lehn C, Lihl C, Roßmann A (2015b) Change of geographical location from Germany (Bavaria) to USA (Arizona) and its effect on H–C–N–S stable isotopes in human hair. Isot Environ Health Stud 51(1):68–79

    CAS  Google Scholar 

  9. Huelsemann F, Flenker U, Koehler K, Schaenzer W (2009) Effect of a controlled dietary change on carbon and nitrogen stable isotope ratios of human hair. Rapid Commun Mass Spectrom 23(16):2448–2454

    CAS  PubMed  Google Scholar 

  10. Petzke KJ, Lemke S (2009) Hair protein and amino acid 13C and 15N abundances take more than 4 weeks to clearly prove influences of animal protein intake in young women with a habitual daily protein consumption of more than 1 g per kg body weight. Rapid Commun Mass Spectrom 23(16):2411–2420

    CAS  PubMed  Google Scholar 

  11. Sachs H (1995) Theoretical limits of the evaluation of drug concentrations in hair due to irregular hair growth. Forensic Sci Int 70(1–3):53–61

    CAS  PubMed  Google Scholar 

  12. Pragst F, Rothe M, Spiegel K, Sporkert F (1998) Illegal and therapeutic drug concentrations in hair segments-a timetable of drug exposure? Forensic Sci Rev 10(2):81–111

    CAS  PubMed  Google Scholar 

  13. Fernández J, Panarello HO, Schobinger J (1999) The Inka mummy from Mount Aconcagua: decoding the geographic origin of the “messenger to the deities” by means of stable carbon, nitrogen, and sulfur isotope analysis. Geoarchaeology 14(1):27–46

    Google Scholar 

  14. O’Connell TC, Hedges RE (1999) Investigations into the effect of diet on modern human hair isotopic values. Am J Phys Anthropol 108(4):409–425

    PubMed  Google Scholar 

  15. Sharp ZD, Atudorei V, Panarello HO, Fernández J, Douthitt C (2003) Hydrogen isotope systematics of hair: archeological and forensic applications. J Archaeol Sci 30(12):1709–1716

    Google Scholar 

  16. Roy DM, Hall R, Mix AC, Bonnichsen R (2005) Using stable isotope analysis to obtain dietary profiles from old hair: a case study from plains Indians. Am J Phys Anthropol 128(2):444–452

    PubMed  Google Scholar 

  17. Wilson AS (2005) Hair as a bioresource in archaeological study. Hair Toxicol: Important bio-monitor:321–344

  18. Wilson AS, Taylor T, Ceruti MC, Chavez JA, Reinhard J, Grimes V, Meier-Augenstein W, Cartmell L, Stern B, Richards MP (2007) Stable isotope and DNA evidence for ritual sequences in Inca child sacrifice. Proc Natl Acad Sci 104(42):16456–16461

    CAS  PubMed  Google Scholar 

  19. Santamaria-Fernandez R, Martínez-Sierra JG, Marchante-Gayon J, García-Alonso JI, Hearn R (2009) Measurement of longitudinal sulfur isotopic variations by laser ablation MC-ICP-MS in single human hair strands. Anal Bioanal Chem 394(1):225–233

    CAS  PubMed  Google Scholar 

  20. Knudson KJ, Pestle WJ, Torres-Rouff C, Pimentel G (2012) Assessing the life history of an Andean traveller through biogeochemistry: stable and radiogenic isotope analyses of archaeological human remains from Northern Chile. Int J Osteoarchaeol 22(4):435–451

    Google Scholar 

  21. Knudson KJ, Peters AH, Cagigao ET (2015) Paleodiet in the Paracas necropolis of Wari Kayan: carbon and nitrogen isotope analysis of keratin samples from the south coast of Peru. J Archaeol Sci 55:231–243

    CAS  Google Scholar 

  22. Williams JS, Katzenberg MA (2012) Seasonal fluctuations in diet and death during the late horizon: a stable isotopic analysis of hair and nail from the central coast of Peru. J Archaeol Sci 39(1):41–57

    CAS  Google Scholar 

  23. Lehn C, Mützel E, Rossmann A (2011) Multi-element stable isotope analysis of H, C, N and S in hair and nails of contemporary human remains. Int J Legal Med 125(5):695–706

    PubMed  Google Scholar 

  24. Lehn C, Graw M (2017) Haare, Zähne und Knochen – Über Isotopenanalysen zu Lebensdaten. In: Rosendahl W, Madea B (eds) Tatorte der Vergangenheit. Archäologie und Forensik, Konrad Theiss Verlag, Darmstadt, pp 85–91

    Google Scholar 

  25. Vautour G, Poirier A, Widory D (2015) Tracking mobility using human hair: what can we learn from lead and strontium isotopes? Sci Justice 55(1):63–71

    PubMed  Google Scholar 

  26. Reynard LM, Burt N, Koon HE, Tuross N (2016) Limits and possibilities in the geolocation of humans using multiple isotope ratios (H, O, N, C) of hair from east coast cities of the USA. Isot Environ Health Stud 52(4–5):498–512

    CAS  Google Scholar 

  27. Britton K, Knecht R, Nehlich O, Hillerdal C, Davis RS, Richards MP (2013) Maritime adaptations and dietary variation in prehistoric Western Alaska: stable isotope analysis of permafrost-preserved human hair. Am J Phys Anthropol 151(3):448–461

    PubMed  Google Scholar 

  28. Britton K, McManus-Fry E, Nehlich O, Richards M, Ledger PM, Knecht R (2016) Stable carbon, nitrogen and sulphur isotope analysis of permafrost preserved human hair from rescue excavations (2009, 2010) at the precontact site of Nunalleq, Alaska. J Archaeol Sci: Reports

  29. Mora A, Arriaza BT, Standen VG, Valdiosera C, Salim A, Smith C (2017) High-resolution palaeodietary reconstruction: amino acid δ 13C analysis of keratin from single hairs of mummified human individuals. Quatern Int 436:96–113

    Google Scholar 

  30. Meier-Augenstein W (2018) Stable isotope forensics: methods and forensic applications of stable isotope analysis, 2nd edn. Wiley, pp 333–400

  31. LeBeau MA, Montgomery MA, Brewer JD (2011) The role of variations in growth rate and sample collection on interpreting results of segmental analyses of hair. Forensic Sci Int 210(1–3):110–116

    PubMed  Google Scholar 

  32. Remien CH, Sussman NL, Adler FR (2013) Mathematical modelling of chronic acetaminophen metabolism and liver injury. Math Med Biol (3):302–317

  33. Hayashi S, Miyamoto I, Takeda K (1991) Measurement of human hair growth by optical microscopy and image analysis. Br J Dermatol 125(2):123–129

    CAS  PubMed  Google Scholar 

  34. Brand WA, Coplen TB, Vogl J, Rosner M, Prohaska T (2014) Assessment of international reference materials for isotope-ratio analysis (IUPAC technical report). Pure Appl Chem 86(3):425–467

    CAS  Google Scholar 

  35. Sieper HP, Kupka HJ, Williams T, Rossmann A, Rummel S, Tanz N, Schmidt HL (2006) A measuring system for the fast simultaneous isotope ratio and elemental analysis of carbon, hydrogen, nitrogen and sulfur in food commodities and other biological material. Rapid Commun Mass Spectrom 20(17):2521–2527

    CAS  PubMed  Google Scholar 

  36. Wassenaar L, Hobson K (2003) Comparative equilibration and online technique for determination of non-exchangeable hydrogen of keratins for use in animal migration studies. Isot Environ Health Stud 39(3):211–217

    CAS  Google Scholar 

  37. Qi H, Coplen TB (2011) Investigation of preparation techniques for δ 2H analysis of keratin materials and a proposed analytical protocol. Rapid Commun Mass Spectrom 25(15):2209–2222

    CAS  PubMed  Google Scholar 

  38. Coplen TB, Qi H (2012) USGS42 and USGS43: human-hair stable hydrogen and oxygen isotopic reference materials and analytical methods for forensic science and implications for published measurement results. Forensic Sci Int 214(1–3):135–141

    CAS  PubMed  Google Scholar 

  39. Bol R, Marsh J, Heaton TH (2007) Multiple stable isotope (18O, 13C, 15N and 34S) analysis of human hair to identify the recent migrants in a rural community in SW England. Rapid Commun Mass Spectrom 21(18):2951–2954

    CAS  PubMed  Google Scholar 

  40. West JB, Bowen GJ, Cerling TE, Ehleringer JR (2006) Stable isotopes as one of nature’s ecological recorders. Trends Ecol Evol 21(7):408–414

    PubMed  Google Scholar 

  41. Ehleringer JR, Bowen GJ, Chesson LA, West AG, Podlesak DW, Cerling TE (2008) Hydrogen and oxygen isotope ratios in human hair are related to geography. Proc Natl Acad Sci 105(8):2788–2793

    CAS  PubMed  Google Scholar 

  42. Birchall J, O’Connell TC, Heaton TH, Hedges RE (2005) Hydrogen isotope ratios in animal body protein reflect trophic level. J Animal Ecol 74(5):877–881

    Google Scholar 

  43. Bowen G. Waterisotopes.org. Accessed 16 January 2018

  44. O’Grady SP, Wende AR, Remien CH, Valenzuela LO, Enright LE, Chesson LA, Abel ED, Cerling TE, Ehleringer JR (2010) Aberrant water homeostasis detected by stable isotope analysis. PLoS One 5(7):e11699

    PubMed  PubMed Central  Google Scholar 

  45. Nehlich O (2015) The application of sulphur isotope analyses in archaeological research: a review. Earth Sci Rev 142:1–17

    CAS  Google Scholar 

  46. Robbins CR (2012) Chemical composition of different hair types. In: Chemical and physical behavior of human hair. Springer, pp 105–176

  47. Auerswald K, Rossmann A, Schäufele R, Schwertl M, Monahan FJ, Schnyder H (2011) Does natural weathering change the stable isotope composition (2H, 13C, 15N, 18O and 34S) of cattle hair? Rapid Commun Mass Spectrom 25(24):3741–3748

    CAS  PubMed  Google Scholar 

  48. Williams LJ, White CD, Longstaffe FJ (2011) Improving stable isotopic interpretations made from human hair through reduction of growth cycle error. Am J Phys Anthropol 145(1):125–136

    CAS  PubMed  Google Scholar 

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Acknowledgments

We are deeply thankful to the two women who were taking part in our study by donating their hair strands and providing information about the travels they had undertaken.

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Correspondence to Christine Lehn.

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Lehn, C., Kalbhenn, E.M., Rossmann, A. et al. Revealing details of stays abroad by sequential stable isotope analyses along human hair strands. Int J Legal Med 133, 935–947 (2019). https://doi.org/10.1007/s00414-018-1866-9

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  • DOI: https://doi.org/10.1007/s00414-018-1866-9

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