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Clinicopathological and cell proliferation evaluation of ameloblastomas and keratocystic odontogenic tumors: a 10 year retrospective study

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Abstract

Odontogenic tumors (OTs) are important lesions of the gnathic bones due to their clinicopathological heterogeneity and variable biological behavior; therefore, epidemiological studies are needed to outline the incidence and behavior of these tumors. To evaluate the incidence and epidemiological profile of ameloblastoma (AMB) and keratocystic odontogenic tumor (KCOT) from an oral pathology service, and correlate morphological findings of these tumors with the immunoexpression of a cellular proliferation marker (Ki-67), a retrospective study (2002–2012) was conducted to characterize demographic, clinical, radiological, and morphological data of AMBs and KCOTs. Then, a representative sample composed of 49 cases of each tumor was selected to perform immunohistochemical (IHC) analysis of Ki-67 through the streptavidin biotin peroxidase technique. For statistical analysis, we used Fisher’s exact test (p < 0.05). A total of 279 OTs were found in the service, in which 91 (32.6%) were AMB and 98 (35 %) were KCOT. Most cases occurred in white women, and the average age of patients with AMB and KCOT was 32 and 33 years, respectively. The maxilla–mandible ratio was 1:6 and 1:3.6 for AMB and KCOT, respectively. Regarding IHC analysis, AMB and KCOT had similar levels of cellular proliferation. However, KCOTs with intense inflammation showed higher Ki-67 expression (p < 0.001). Recurrent cases had similar Ki-67 immunoexpression. The demographic profile of the studied tumors corroborates with data reported in the literature, and the levels of cellular proliferation were similar in both tumors, although the inflammation seems to induce a differential proliferative behavior in KCOT.

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Acknowledgments

We would like to thank the support given by the Brazilian National Council of Technological and Scientific Development (CNPq).

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Authors and Affiliations

  1. Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil

    Leorik Pereira da Silva

  2. Genomics and Molecular Biology Laboratory, A. C. Camargo Cancer Centre, São Paulo, SP, Brazil

    Marianna Sampaio Serpa

  3. Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

    Thalita Santana

  4. Oral Pathology, Biological Sciences Academic Center, School of DentistryFederal University of Campina Grande, Patos, Paraíba, Brazil

    George João Ferreira do Nascimento

  5. Oral Pathology, School of Dentistry, University of Pernambuco, Camaragibe, Pernambuco, Brazil

    Emanuel Sávio de Souza Andrade & Ana Paula Veras Sobral

  6. Programa de Pós-Graduação em Patologia Oral, Universidade Federal do Rio Grande do Norte, UFRN, Av. Salgado Filho, 1787, Lagoa Nova, Natal/RN, CEP, 59056-000, Brazil

    Leorik Pereira da Silva

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  1. Leorik Pereira da Silva
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Correspondence to Leorik Pereira da Silva.

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All authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study involving the patient were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For this type of study, formal consent is not required.

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Informed consent was obtained from the patients in this study.

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da Silva, L.P., Serpa, M.S., Santana, T. et al. Clinicopathological and cell proliferation evaluation of ameloblastomas and keratocystic odontogenic tumors: a 10 year retrospective study. Eur Arch Otorhinolaryngol 274, 1089–1095 (2017). https://doi.org/10.1007/s00405-016-4348-z

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