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Histologıcal results of HPV genotyping from a colposcopy center

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To investigate the role of partial human papillomavirus (HPV) genotyping tests in predicting the diagnosis of high-grade cervical intraepithelial lesion and cancer (HSIL +) as a result of colposcopic histopathology.

Materials and methods

The study included 2872 patients who presented at our colposcopy unit between January 1, 2015 and December 31, 2019 and underwent colposcopy for the first time. The patients were compared in terms of HSIL + results as HPV 16/18 and HPV other type positive groups.

Results

HSIL + was determined at the rate of 22.3% in the HPV 16/18 group and at 7.0% in the HPV Other group, and the difference was statistically significant (p = 0.000). HPV 16/18 types were found to be responsible for 84.8% of cervical cancers and 83.5% of HSIL and worse cases.

Conclusion

Partial HPV 16/18 genotyping is an effective strategy in the triage of HPV-positive women. HPV type identification consistent with the epidemiology of HPV types in HSIL + cases in the screened population, and the age-appropriate use of primary HPV tests will determine the sensitivity and cost effectiveness of screening.

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Data availability

Data will be provided if asked.

Abbreviations

ASCCP:

American Society for Colposcopy and Cervical Pathology

CIN:

Cervical intraepithelial lesion

AGC:

Atypical glandular cells

ASCUS:

Atypical squamous cells of undetermined significance

LSIL:

Low-grade squamous intraepithelial lesion

SIL:

Squamous intraepithelial lesion in which low- and high-grade squamous intraepithelial lesion cannot be decided

ASC-H:

Atypical squamous cells, cannot exclude high-grade intraepithelal lesion

HSIL:

High-grade squamous intraepithelial lesion

HSIL + :

Patients with high-grade squamous intraepithelial lesion, adenocarcinoma in situ, cervical cancer,and non-cervical cancer

ECC:

Endocervical curettage

LEEP:

Loop electrosurgical excision procedure

References

  1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 71:209–249

    Article  PubMed  Google Scholar 

  2. World Health Organization: “Draft Global Strategy towards eliminating cervical cancer as a public health problem” https://www.who.int/publications/m/item/draft-global-strategy-towards-eliminating-cervical-cancer-as-a-public-health-problem. [Accessed 20 February 2022]

  3. Walboomers JM, Jacobs MV, Manos MM, Bosch FX, Kummer JA, Shah KV, Snijders PJ, Peto J, Meijer CJ, Munoz N (1999) Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol 189:12–19

    Article  CAS  PubMed  Google Scholar 

  4. Smith JS, Lindsay L, Hoots B, Keys J, Franceschi S, Winer R, Glifford GM (2007) Human papillomavirus type distribution in invasive cervical cancer and high-grade cervical lesions: a meta-analysis update. Int J Cancer 121:621–632

    Article  CAS  PubMed  Google Scholar 

  5. Munoz N, Bosch FX, de Sanjose S, Herrero R, Castellsague X, Shah KV, Snijders PJ, Meier CJ, International agency for research on cancer multicenter cervical cancer study group (2003) Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med 348(518):527

    Google Scholar 

  6. Wingo PA, Cardinez CJ, Landis SH, Greenlee RT, Ries LA, Anderson RN, Thun MJ (2003) Long-term trends in cancer mortality in the United States, 1930–1998. Cancer 97:3133–3275

    Article  PubMed  Google Scholar 

  7. Ronco G, Dillner J, Elfström KM, Tunesi S, Snijders PJ, Arbyn M, Kitchener H, Segnan N, Gilham C, Giorgi-Rossi P, Berkhof J, Peto J, Meijer CJ, International HPV screening working group (2014) Efficacy of HPV-based screening for prevention of invasive cervical cancer: follow-up of four European randomised controlled trials. The Lancet 383(524):532

    Google Scholar 

  8. Gultekin M, Zayifoglu Karaca M, Kucukyildiz I, Dundar S, Boztas G, Semra Turan H, Hacikamiloglu E, Murtuza K, Keskinkilic B, Sencan I (2018) Initial results of population based cervical cancer screening program using HPV testing in one million Turkish women. Int J Cancer 142:1952–1958

    Article  CAS  PubMed  Google Scholar 

  9. Aoyama-Kikawa S, Fujita H, Hanley SJB, Kasamo M, Kikuchi K, Torigoe T, Matsuno Y, Tamakoshi A, Sasaki T, Matsuura M, Kato Y, Dong P, Watari H, Saito T, Sengoku K, Sakuragi N (2018) Comparison of human papillomavirus genotyping and cytology triage, COMPACT Study: design, methods and baseline results in 14 642 women. Cancer Sci 2018(109):2003–2012

    Article  Google Scholar 

  10. Hashim D, Engesæter B, Skare GB, Castle PE, BjØrge T, Tropé A, Nygård M (2020) Real-world data on cervical cancer risk stratification by cytology and HPV genotype to inform the management of HPV-positive women in routine cervical screening. Br J Cancer 122:1715–1723

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Torres-Ibarra L, Cuzick J, Lorincz AT, Spiegelman D, Lazcano-Ponce E, Franco EL, Moscicki AB, Mahmud SM, Wheeler CM, Rivera-Paredez B, Hernández-López R, León-Maldonado L, Salmerón J, FRIDA Study Group (2019) Comparison of HPV-16 and HPV-18 genotyping and cytological testing as triage testing within human papillomavirus-based screening in Mexico. JAMA Netw Open 2:e1915781–e1915781

    Article  PubMed  PubMed Central  Google Scholar 

  12. Huh WK, Williams E, Huang J, Bramley T, Poulios N (2015) Cost effectiveness of human papillomavirus-16/18 genotyping in cervical cancer screening. Appl Health Econ Health Policy 13:95–107

    Article  PubMed  Google Scholar 

  13. Xu L, Benoy I, Cuschieri K, Poljak M, Bonde J, Arbyn M (2019) Accuracy of genotyping for HPV16 and 18 to triage women with low-grade squamous intraepithelial lesions: a pooled analysis of VALGENT studies. Expert Rev Mol Diagn 19:543–551

    Article  CAS  PubMed  Google Scholar 

  14. Bonde JH, Sandri MT, Gary DS, Andrews JC (2020) Clinical utility of human papillomavirus genotyping in cervical cancer screening: a systematic review. J Low Genit Tract Dis 24:1–13

    Article  PubMed  Google Scholar 

  15. Sung YE, Ki EY, Lee YS, Hur SY, Lee A, Park JS (2016) Can human papillomavirus (HPV) genotyping classify non-16/18 high-risk HPV infection by risk stratification? J of Gynecol Oncol 27:e56

    Article  Google Scholar 

  16. Clifford GM, Smith JS, Plummer M, Munoz N, Franceschi S (2003) Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysis. Br J Cancer 88:63–73

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Chiang YC, Cheng WF, Chen YL, Chang MC, Hsieh CY, Lin MC, Chen CA (2013) High-risk human papillomavirus, other than type 16/18, in predominantly older Taiwanese women with high-grade cervical preinvasive lesions. Taiwan J Obstet Gynecol 52:222–226

    Article  PubMed  Google Scholar 

  18. Tjalma WA, Fiander A, Reich O, Powell N, Am N, Kirschner B et al (2013) Differences in human papillomavirus type distribution in high-grade cervical intraepithelial neoplasia and invasive cervical cancer in Europe. Int J Cancer 132:854–867

    Article  CAS  PubMed  Google Scholar 

  19. Veldhuijzen NJ, Berkhof J, Gillio-Tos A, De Marco L, Carozzi F, Del Mistro A, Snijders PJ, Meijer CJ, Ronco G (2015) The age distribution of type-specific high-risk human papillomavirus incidence in two population-based screening trials. Cancer Epidemiol Prev Biomark 24:111–118

    Article  Google Scholar 

  20. Arbyn M, Depuydt C, Benoy I, Bogers J, Cuschieri K, Schmitt M, Pawlita M, Geraets D, Heard I, Gheit T, Tommasino M, Poljak M, Bonde J, Quint W (2016) VALGENT: a protocol for clinical validation of human papillomavirus assays. J Clin Virol 76:14–21

    Article  Google Scholar 

  21. Gultekin M, Dundar S, Keskinkilic B, Turkyilmaz M, Ozgul N, Yuce K, Kara F (2020) How to triage HPV positive cases: Results of four million females. Gynecol Oncol 158:105–111

    Article  CAS  PubMed  Google Scholar 

  22. Perkins RB, Guido RS, Castle PE, Chelmow D, Einstein MH, Garcia F, Huh WK, Kim JJ, Moscicki AB, Nayar R, Saraiya M, Sawaya GF, Wentzensen N, Schiffman M, ASCCP risk-based management consensus guidelines committee (2020) 2019 ASCCP risk-based management consensus guidelines for abnormal cervical cancer screening tests and cancer precursors. J Low Genit Tract Dis 24:102–131

    Article  PubMed  PubMed Central  Google Scholar 

  23. Huh WK, Ault KA, Chelmow D, Davey DD, Goulart RA, Garcia FAR, Kinney WK, Massad LS, Mayeaux EJ, Saslow D, Schiffman M, Wentzensen N, Lawson HW, Einstein MH (2015) Use of primary high-risk human papillomavirus testing for cervical cancer screening: interim clinical guidance. Obstet Gynecol 125:330–337

    Article  PubMed  Google Scholar 

  24. Turkish Cancer Statistics 2017: https://hsgm.saglik.gov.tr/depo/birimler/kanser-db/istatistik/Turkiye_Kanser_statistikleri_2017.pdf

  25. Barra F, Della Corte L, Noberasco G, Foreste V, Riemma G, Di Filippo C, Ferrero S (2020) Advances in therapeutic vaccines for treating human papillomavirus-related cervical intraepithelial neoplasia. J Obstet Gynaecol Res 46(7):989–1006

    Article  PubMed  Google Scholar 

  26. Karafillakis E, Peretti-Watel P, Verger P, Chantler T, Larson HJ (2022) “We don’t have the same bodies; we don’t react the same way”: mothers and adolescent girls’ perceptions of the risks and benefits of HPV vaccination in France. Hum Vaccin Immunother 18(1):2036555

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Riemma G, Schettino MT, Munno GM, Fasulo DD, Sandullo L, Amabile E, Torella M (2022) Echinacea angustifolia and echinacea purpurea supplementation combined with vaginal hyaluronic acid to boost the remission of cervical low-grade squamous intraepithelial lesions (L-SILs): a randomized controlled trial. Medicina 58(5):646

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors would like to thank the pathologists working in the Pathology Departmant of the hospital for their support and clinical work.

Funding

None to declare.

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Authors and Affiliations

  1. Department of Gynecologic Oncology, University of Health Sciences Turkey, Etlik Zubeyde Hanim Women’s Health Education and Research Hospital, Ankara, Turkey

    Sevgi Koc, Dilek Yuksel & Fulya Kayikcioglu

  2. Department of Gynecology and Obstetrics, University of Health Sciences Turkey, Etlik Zubeyde Hanim Women’s Health Education and Research Hospital, Ankara, Turkey

    Tugba Kinay & Derya Burkankulu

Authors
  1. Sevgi Koc
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  2. Dilek Yuksel
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  3. Tugba Kinay
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  5. Fulya Kayikcioglu
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Contributions

Study design: SK; data management: SK, DY,TK, DB; manuscript writing/editing: SK; literature Search: SK, DY,TK, DB; statistical analysis: SK, DY; drafted the paper: SK, FK, DY,TK; colposcopic work-up: SK, FK.

Corresponding author

Correspondence to Sevgi Koc.

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Conflict of interest

The authors have no conflict of interests to declare.

Ethical approval

This study was approved by the Institutional Review Board of the University of Health Sciences Turkey, Etlik Zubeyde Hanim Women’s Health Training and Research Hospital (Date: 21.04.2020, No: 06/10).

Informed consent

All patients provided informed consent before the colposcopy procedure.

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Koc, S., Yuksel, D., Kinay, T. et al. Histologıcal results of HPV genotyping from a colposcopy center. Arch Gynecol Obstet 308, 193–200 (2023). https://doi.org/10.1007/s00404-022-06879-5

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  • DOI: https://doi.org/10.1007/s00404-022-06879-5

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