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A simple, noninvasive and efficient method for transdermal delivery of siRNA

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Abstract

Effective delivery of therapeutic agents is the most challenging hurdle in the use of RNA interference for research and in the clinic. Here, we assessed whether a short synthetic peptide, ACSSSPSKHCG (TD-1), could be transported through rat footpad (follicle-free) skin and efficiently deliver small interfering RNA (siRNA) to knock down a target gene. Fluorescence microscopy revealed that topical co-administration of FITC-labeled TD-1 and FAM-labeled siRNA distributed uniformly from the epidermis to the subcutaneous tissue of rat footpad skin. Transmission electron microscopy revealed the absence of cell–cell junctions and enlarged spaces between epithelial cells in the TD-1-treated footpad skin. TD-1 delivery of anti-GAPDH siRNA significantly reduced the level of GAPDH in 72 h. TD-1 can create a transient opening in non-follicle rat skin for delivery of siRNA and reveal a novel mechanism of transdermal delivery of TD-1 and siRNA into the epidermis for gene knockdown. The system might have potential for siRNA delivery in skin for drug therapy.

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Abbreviations

RNAi:

RNA interference

siRNAs:

Small interfering RNAs

FITC:

Fluorescein isothiocyanate

HPLC:

High-performance liquid chromatography

NC:

Negative control

TEM:

Transmission electron microscopy

FAM:

Carboxyfluorescein

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 30801196, No. 81071577) and the Natural Science Foundation of Guangdong Province (No. 9151008901000141).

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, No. 57, ChangPing Road, ShanTou City, Guangdong Province, China

    Chang-Min Lin, Sen Wang & Yu Li

  2. Emergency Department, Second Affiliated Hospital, Shantou University Medical College, ShanTou City, China

    Keng Huang

  3. Department of Histology and Embryology, Shantou University Medical College, ShanTou City, China

    Chang-Min Lin, Yang Zeng & Xian-Cai Chen

Authors
  1. Chang-Min Lin
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  2. Keng Huang
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  3. Yang Zeng
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  4. Xian-Cai Chen
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  5. Sen Wang
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  6. Yu Li
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Corresponding author

Correspondence to Yu Li.

Additional information

C-M. Lin and K. Huang contributed equally to this work.

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Lin, CM., Huang, K., Zeng, Y. et al. A simple, noninvasive and efficient method for transdermal delivery of siRNA. Arch Dermatol Res 304, 139–144 (2012). https://doi.org/10.1007/s00403-011-1181-5

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  • DOI: https://doi.org/10.1007/s00403-011-1181-5

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