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Fucoidan suppresses IgE production in peripheral blood mononuclear cells from patients with atopic dermatitis

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Abstract

We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production in B cells from mice spleen in vitro and ovalbumin-sensitized mice in vivo. In this study, we examined the effect of fucoidan on IgE production in human peripheral blood mononuclear cells (PBMC) in vitro. PBMC, obtained from healthy donors or patients with atopic dermatitis (AD) with high levels of serum IgE, were cultured with IL-4 and anti-CD40 antibody in the presence or absence of fucoidan. Fucoidan significantly reduced IgE production in PBMC without affecting cell proliferation and IFN-γ production. Fucoidan also inhibited immunoglobulin germline transcripts of B cells in PBMC, and decreased the number of IgE-secreting cells. The inhibitory effects of fucoidan were similarly observed for both PBMC from patients with AD and those with healthy donors. Our findings indicate that fucoidan suppresses IgE induction by inhibiting immunoglobulin class-switching to IgE in human B cells, even after the onset of AD.

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Abbreviations

PBMC:

Peripheral blood mononuclear cells

AD:

Atopic dermatitis

FcεRI:

High affinity IgE receptor

mAb:

Monoclonal antibody

OVA:

Ovalbumin

RT-PCR:

Reverse transcription-polymerase chain reaction

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

We thank Ms Kazue Uchida and Ms Kaori Ishii for the expert technical assistances, and Dr Faiz Kermani for critical review of the manuscript.

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Authors and Affiliations

  1. Division of Molecular Medical Science, Department of Dermatology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan

    Kazumasa Iwamoto, Takaaki Hiragun, Shunsuke Takahagi, Yuhki Yanase, Satoshi Morioke, Shoji Mihara, Yoshikazu Kameyoshi & Michihiro Hide

  2. Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan

    Takaaki Hiragun

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  1. Kazumasa Iwamoto
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  2. Takaaki Hiragun
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  3. Shunsuke Takahagi
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  4. Yuhki Yanase
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  5. Satoshi Morioke
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  6. Shoji Mihara
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  7. Yoshikazu Kameyoshi
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  8. Michihiro Hide
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Correspondence to Michihiro Hide.

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Iwamoto, K., Hiragun, T., Takahagi, S. et al. Fucoidan suppresses IgE production in peripheral blood mononuclear cells from patients with atopic dermatitis. Arch Dermatol Res 303, 425–431 (2011). https://doi.org/10.1007/s00403-010-1115-7

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  • DOI: https://doi.org/10.1007/s00403-010-1115-7

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