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Effects of acitretin on proliferative inhibition and RANTES production of HaCaT cells

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Abstract

Acitretin is one of the first line drugs to treat psoriasis, though the mechanisms are not fully clear. It has been reported that psoriatic keratinocytes secreted high level of RANTES, which can induce chemotaxis and activation of T cells, thus may play an important role in the pathogenesis of psoriasis. However, the effect of acitretin on RANTES production of human keratinocytes and its possible signaling are unclear. We observed a significant inhibition of acitretin on proliferation of human keratinocytes cell line (HaCaT cells) by MTT assay. The inhibition rate of HaCaT cells growth increased gradually from 13.70 to 67.73% with acitretin concentration rising from 0.01 to 50 μmol/L. In addition, RANTES expression detected in supernatant of HaCaT cells stimulated with TNF-α and IFN-γ reduced 25, 18 and 12%, respectively, when cultured with 0.1, 1 and 5 μmol/L acitretin. Furthermore, 1 μmol/L acitretin significantly decreased RANTES mRNA level. Finally, acitretin decreased the expressions of signal transducer and activator of transcription 1 (STAT1) and nuclear factor kappa B (NFκB) in nuclei of HaCaT cells stimulated with TNF-α and IFN-γ, which were determined by immunocytochemistry and western blot. It suggests that acitretin can inhibit proliferation and RANTES production of human epidermal keratinocytes, and the latter may be related to the inhibition of nuclear translocations of STAT1 and NFκB.

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Authors and Affiliations

  1. Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu, China

    Min Zhang & Yuping Ran

  2. Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China

    Ling Zhu, Yunxia Yang & Liao Liu

  3. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China

    Yun Feng

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  1. Min Zhang
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  2. Ling Zhu
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  3. Yun Feng
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  4. Yunxia Yang
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  5. Liao Liu
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  6. Yuping Ran
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Correspondence to Yuping Ran.

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Zhang, M., Zhu, L., Feng, Y. et al. Effects of acitretin on proliferative inhibition and RANTES production of HaCaT cells. Arch Dermatol Res 300, 575–581 (2008). https://doi.org/10.1007/s00403-008-0872-z

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  • DOI: https://doi.org/10.1007/s00403-008-0872-z

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