Abstract
N-methyl-d-aspartate receptors (NMDAR) can regulate the intracellular calcium concentration of keratinocytes (KC) and seem to be important for their growth and differentiation. The objective of this study was to identify the subtype(s) of this receptor expressed by KC in vitro. The mRNA was isolated from primary cultures of KC as well as from a KC cell line (HaCaT) and expression of the NMDAR subtypes determined by using RT-PCR. At the mRNA level, we found expression of only the constant NMDAR1 as well as the subtype NMDAR2D. In contrast to the other subtypes of NMDAR, NMDAR2D is characterized by low influence of magnesium to the receptor function. This characteristic is consistent with previously published functional investigations in KC. The identification of the NMDAR2D subtype in KC may be of value for the development of new therapeutic approaches.
Similar content being viewed by others
References
Danysz W, Parsons CG (1998) Glycine and N-methyl-D-aspartate receptors: physiological significance and possible therapeutic applications. Pharmacol Rev 50:597–664
Fischer M, Glanz D, William T, Klapperstück T, Wohlrab J, Marsch WC (2004) N-methyl-D-aspartate receptors influence the intracellular calcium concentration of keratinocytes. Exp Dermatol 13:512–519
Fischer M, William T, Helmbold P, Wohlrab J, Marsch WC (2004) Expression of epidermal N-methyl-D-aspartate receptors (NMDAR1) depends on formation of the granular layer-analysis in diseases with parakeratotic cornification. Arch Dermatol Res 296:157–162
Fuziwara S, Inoue K, Denda M (2003) NMDA-type glutamate receptor is associated with cutaneous barrier homeostasis. J Invest Dermatol 120:1023–1029
Hess SD, Daggett LP, Deal C, Lu CC, Johnson EC, Velicelebi G (1998) Functional characterization of human N-methyl-D-aspartate subtype 1A/2D receptors. J Neurochem 70:1269–1279
Hitchcock IS, Skerry TM, Howard MR, Genever PG (2003) NMDA receptor-mediated regulation of human megakaryocytopoiesis. Blood 102:1254–1259
Lansdown ABG (2002) Calcium: a potential central regulator in wound healing in the skin. Wound Rep Reg 10:271–285
Lynch DR, Anegawa NJ, Verdoorn T, Pritchett DB (1994) N-methyl-D-aspartate receptors: different subunit requirements for binding of glutamate antagonists, glycine antagonists, and channel-blocking agents. Mol Pharmacol 45:540–545
Misra C, Brickley SG, Wyllie DJ, Cull-Candy SG (2000) Slow deactivation kinetics of NMDA receptors containing NR1 and NR2D subunits in rat cerebellar Purkinje cells. J Physiol 525:299–305
Morhenn VB, Waleh NS, Mansbridge JN, Unson D, Zolotorev A, Cline P, Toll L (1994) Evidence for an NMDA receptor subunit in human keratinocytes and rat cardiocytes. Eur J Pharmacol 268:409–414
Morhenn VB, Murakami M, O’Grady T, Nordberg J, Gallo RL (2004) Characterization of the expression and function of N-methyl-D-aspartate receptor in keratinocytes. Exp Dermatol 13:505–511
Nahm WK, Philpot BD, Adams MM, Badiavas EV, Zhou LH, Butmarc J, Bear MF, Falanga V (2004) Significance of N-methyl-D-aspartate (NMDA) receptor-mediated signalling in human keratinocytes. J Cell Physiol 200:309–317
Stephenson FA (2001) Subunit characterization of NMDA receptors. Curr Drug Targets 2:233–239
Acknowledgement
This study was supported by the BMBF (FKZ01ZZ104) and the Wilhelm-Roux-Programm (NBL3, FKZ 10/31).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fischer, M., Fiedler, E., Seidel, C. et al. Cultivated keratinocytes express N-methyl-d-aspartate receptors of the NMDAR2D type. Arch Dermatol Res 297, 316–318 (2006). https://doi.org/10.1007/s00403-005-0625-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00403-005-0625-1