Skip to main content
SpringerLink
Log in

The effects of Peptide Mel4-coated titanium plates on infection rabbits after internal fixation of open fractures

  • Orthopaedic Surgery
  • Published:
Archives of Orthopaedic and Trauma Surgery Aims and scope Submit manuscript

Abstract

Introduction

Infection after internal fixation surgery is an orthopedic serious complication which affect the fracture healing. The primary objective of this study was to verify the effects of a Peptide Mel4-coated titanium plate applied in the treatment of infection after internal fixation of open fracture.

Materials and methods

Eighty-eight rabbits were intravenously inoculated with Staphylococcus aureus or Pseudomonas aeruginosa suspensions. Bacterial cultures were obtained from titanium plates at 1st, 3rd, 5th, 7th and 9th days. Blood samples were collected at 1st, 3rd, 5th, 7th and 9th days after the infection.

Results

Mel4-coated titanium plates have significant inhibitory effects on Staphylococcus aureus and Pseudomonas aeruginosa (P < 0.05), and there are significant differences in serum IL-1 and TNF-α levels (P < 0.05).

Conclusion

We suggest that the use of Mel4-coated titanium plates may be a promising way to control postoperative infection of open fracture in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Zalavras CG, Patzakis MJ, Holtom P (2004) Local antibiotic therapy in the treatment of open fractures and osteomyelitis. ClinOrthopRelat Res 427:86–93

    Google Scholar 

  2. Tribble DR, Lewandowski LR, Potter BK, Petfield JL, Stinner DJ, Ganesan A, Krauss M, Murray CK and Trauma Infectious Disease Outcomes Study G (2018) Osteomyelitis risk factors related to combat trauma open tibia fractures: a case-control analysis. J Orthop Trauma 32(9):e344

    Article  Google Scholar 

  3. Ikem ICF, Oginni LM, Bamgboye EA, Ako-Nai AK, Onipade AO (2004) The bacteriology of open fractures in Ile-Ife, Nigeria. Niger J Med 13(4):359–365

    PubMed  Google Scholar 

  4. Gupta P, Vishwakarma AK, Gupta DK, Agarwal S, Singh N (2017) Comparative evaluation of results after internal fixation of fracture clavicle by titanium elastic nailing system/plate. J Bone Join Dis 32(2):10–16

    Google Scholar 

  5. Doshi P, Gopalan H, Sprague S, Pradhan C, Kulkarni S, Bhandari M (2017) Incidence of infection following internal fixation of open and closed tibia fractures in India (INFINITI): a multi-centre observational cohort study. BMC MusculoskeletDisord 18(1):156

    Article  Google Scholar 

  6. Papakostidis C, Kanakaris NK, Pretel J, Faour O, Giannoudis PV (2011) Prevalence of complications of open tibial shaft fractures stratified as per the Gustilo-Anderson classification. Injury 42(12):1408–1415

    Article  Google Scholar 

  7. Lyndon JA, Boyd BJ, Birbilis N (2014) Metallic implant drug/device combinations for controlled drug release in orthopaedic applications. J Control Release 179:63–75

    Article  CAS  Google Scholar 

  8. Marr AK, Gooderham WJ, Hancock REW (2006) Antibacterial peptides for therapeutic use: obstacles and realistic outlook. CurrOpinPharmacol 6(5):468–472

    CAS  Google Scholar 

  9. Dutta D, Zhao T, Cheah KB, Holmlund L and Willcox MDP (2017) Activity of a melimine derived peptide Mel4 against Stenotrophomonas, Delftia, Elizabethkingia, Burkholderia and biocompatibility as a contact lens coating. Cont Lens Anterior Eye S1367048416301163

  10. Yasir M, Dutta D, Willcox MDP (2019a) Comparative mode of action of the antimicrobial peptide melimine and its derivative Mel4 against Pseudomonas aeruginosa. Sci Rep 9(1):7063

    Article  Google Scholar 

  11. Hochuli-Vieira E, Gabrielli MAC, Pereira-Filho VA, Gabrielli MFR, Padilha JG (2005) Rigid internal fixation with titanium versus bioresorbableminiplates in the repair of mandibular fractures in rabbits. Int J Oral MaxillofacSurg 34(2):167–173

    Article  CAS  Google Scholar 

  12. Portol SM, Austin F, Nos-Barber S, Paterson C, Refojo MF (1995) Effect of poloxamer 407 on the adherence of Pseudomonas aeruginosa to corneal epithelial cells. Cornea 14(1):56–61

    Google Scholar 

  13. Zhang H, Wang J, Liu Z, Yang S, Ding S, Gu X (2019) Application of GGCX combined with inflammatory factors IL-1 and TNF-α in the evaluation of knee osteoarthritis. J Biomater Tissue Eng 9(1):134–138

    Article  Google Scholar 

  14. Nigam A, Gupta D, Sharma A (2014) Treatment of infectious disease: beyond antibiotics. Microbiol Res 169(9–10):643–651

    Article  CAS  Google Scholar 

  15. Nseir S, Di Pompeo C, Soubrier S, Delour P, Lenci H, Roussel-Delvallez M, Onimus T, Saulnier F, Mathieu D, Durocher A (2005) First-generation fluoroquinolone use and subsequent emergence of multiple drug-resistant bacteria in the intensive care unit. Crit Care Med 33(2):283–289

    Article  CAS  Google Scholar 

  16. Yi L, Dang J, Zhang L, Wu Y, Liu B, Lü X (2016) Purification, characterization and bactericidal mechanism of a broad spectrum bacteriocin with antimicrobial activity against multidrug-resistant strains produced by Lactobacillus coryniformis XN8. Food Control 67:53–62

    Article  CAS  Google Scholar 

  17. Pompilio A, Scocchi M, Pomponio S, Guida F, Primio AD, Fiscarelli E, Gennaro R, Bonaventura GD (2011) Antibacterial and anti-biofilm effects of cathelicidin peptides against pathogens isolated from cystic fibrosis patients. Peptides 32(9):1807–1814

    Article  CAS  Google Scholar 

  18. Srour M, Inaba K, Okoye O, Chan C, Skiada D, Schnüriger B, Trump M, Lam L, Demetriades D (2015) Prospective evaluation of treatment of open fractures: effect of time to irrigation and debridement. JAMA Surg 150(4):332–336

    Article  Google Scholar 

  19. Singh J, Rambani R, Hashim Z, Raman R, Sharma HK (2012) The relationship between time to surgical debridement and incidence of infection in grade III open fractures. Strategies Trauma Limb Reconstr 7(1):33–37

    Article  Google Scholar 

  20. Brook I, Frazier EH (1998) Aerobic and anaerobic microbiology of infection after trauma. Am J Emerg Med 16(6):585–591

    Article  CAS  Google Scholar 

  21. Kojouharoff G, Hans W, Obermeier F, Mannel DN, Andus T, Scholmerich J, Gross V, Falk W (1997) Neutralization of tumour necrosis factor (TNF) but not of IL-1 reduces inflammation in chronic dextran sulphate sodium-induced colitis in mice. ClinExpImmunol 107(2):353–358

    CAS  Google Scholar 

  22. Yasir M, Dutta D, Willcox MDP (2019b) Mode of action of the antimicrobial peptide Mel4 is independent of Staphylococcus aureus cell membrane permeability. PLoS ONE 14(7):e0215703

    Article  CAS  Google Scholar 

Download references

Funding

This study was supported by National Natural Science Foundations of China (81702179) and Funding from Young Talent Development Plan of Changzhou Health Commission (CZQM2020059).

Author information

Authors and Affiliations

  1. Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, Changzhou, 213000, China

    Su Zhang, Xindie Zhou, Ting Liu & Yong Huang

  2. Department of Orthopedic Surgery, The Second Affiliated Hospital of Jiaxing University, Jiaxing, 314000, China

    Jin Li

Authors
  1. Su Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xindie Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ting Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yong Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yong Huang or Jin Li.

Ethics declarations

Conflict of interest

All authors declare that they have no conflicts of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, S., Zhou, X., Liu, T. et al. The effects of Peptide Mel4-coated titanium plates on infection rabbits after internal fixation of open fractures. Arch Orthop Trauma Surg 142, 729–734 (2022). https://doi.org/10.1007/s00402-020-03694-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00402-020-03694-y

Keywords

Search

Navigation