Abstract
Introduction
We investigated the effects of extracorporeal shock waves on cytotoxicity and on the proliferation of human chondrocytes and ovine bone marrow stromal cells.
Materials and methods
Isolated cells were cultured to confluence, and 500 shock waves were applied at energy flux densities of 0, 0.02, 0.06, and 0.17 mJ/mm2 for the cytotoxicity assay. The same energies at 100, 500, and 1000 impulses were used for the proliferation assay.
Results
Although bone marrow stromal cells revealed a dose- and impulse-dependent increase in the proliferation rate, no significant differences were found. Chondrocytes had less proliferative potential than untreated control groups. In the experimental set-up using 1000 impulses, proliferation was even higher in the control group. Both types of cells revealed a dose-dependent increase in cytotoxicity in the lactate dehydrogenase (LDH) assay.
Conclusion
As femoral head necrosis, osteochondritis dissecans, and similar disorders are increasingly treated with shock waves, their effect on human cartilage and chondrocytes deserves attention. We recommend further in vitro experiments with bone marrow stromal cells, as the latter might play an important role in the presumed multifactorial osteogenetic mechanism of shock waves due to their pluripotent character.
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References
Delius M, Draenert K, Aldiek Y (1995) Biological effects of shock waves: in vivo effect of high energy pulses on rabbit bone. Ultrasound Med Biol 21:1219–1225
Ekkernkamp A, Bosse A, Haupt G, Pommer A (1992) Der Einfluss der extracorporalen Stoßwellen auf die standardisierte Tibiafraktur am Schaf. In: Ittel T, Sieberth G, Matthiaß H (eds) Aktuelle Aspekte der Osteologie. Springer, Berlin Heidelberg New York, pp 307–310
Forriol F, Solchaga L, Moreno JL et al (1994) The effect of shock waves on mature and healing cortical bone. Int Orthop 18:325–329
Haake M, Wessel C, Wilke A (1999) Einwirkung extrakorporaler Stoßwellen (ESW) auf humane Knochenmarkszellkulturen. Biomed Tech 44:278–282
Haupt G, Haupt A, Ekkernkamp A et al (1992) Influence of shock waves on fracture healing. J Urol 39:529–532
Heller KD, Niethard FU (1998) Der Einsatz der extrakorporalen Stoßwellentherapie in der Orthopädie-eine Metaanalyse. Z Orthop 136:390–401
Ikeda K, Tomita K, Takayama K (1999) Application of extracorporeal shock waves on bone: preliminary report. J Trauma 47:946–950
Johannes EJ, Kaulesar-Sukul DM, Matura E (1994) High energy shock waves for the treatment of nonunions: an experiment on dogs. J Surg Res 57:246–252
Kubista B, Trieb K, Blahovec H, Kotz R, Micksche M (2002) Hyperthermia increases the susceptibility of chondro- and osteosarcoma cells to natural killer cell mediated lysis. Anticancer Res 22:789–792
Ludwig J, Lauber S, Lauber HJ, Dreisilker U, Raedel R, Hotzinger H (2001) High-energy shock wave treatment of femoral head necrosis in adults. Clin Orthop 387:119–126
Marx SI, Thiele R (2002) New indications in ESWT; presentation at the 2nd 3-nations meeting of Austrian, Swiss and German Societies for Orthopaedic ESWT, April 2002, Linz, Austria
Rompe JD, Rosendahl T, Schöllner C, Theis C (2001) High-energy extracorporeal shock wave treatment of nonunions. Clin Orthop 387:102–111
Schaden W, Fischer A, Sailler A (2001) Extracorporeal shock wave therapy of non-union or delayed osseous union. Clin Orthop 387:90–94
Valchonov VD, Michailov P (1991) High energy shock waves in the treatment of delayed and nonunions of fractures. Int Orthop 15:181–184
Väterlein N, Lüssenhop S, Hahn M, Delling G, Meiss AL (2000) The effect of extracorporeal shock waves on joint cartilage—an in vivo study in rabbits. Arch Orthop Trauma Surg 120:403–406
Vogel JC, Hopf P, Eysel JD, Rompe JD (1997) Application of extracorporeal shock waves in the treatment of pseudarthrosis of the lower extremity—preliminary results. Arch Orthop Trauma Surg 116:480–483
Wang FS, Wang CJ, Huang HJ, Chung H, Chen RF, Yang KD (2001) Physical shock wave mediates membrane hyperpolarization and Ras activation for osteogenesis in human bone marrow stromal cells. Biochem Biophys Res Commun 287:648–655
Yeaman LD, Jerome CP, McCullough DL (1989) Effects of shock waves on the structure and growth of the immature rat epiphysis. J Urol 141:670–674
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The authors declare that the experiments comply with the current laws.
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Dorotka, R., Kubista, B., Schatz, KD. et al. Effects of extracorporeal shock waves on human articular chondrocytes and ovine bone marrow stromal cells in vitro. Arch Orthop Trauma Surg 123, 345–348 (2003). https://doi.org/10.1007/s00402-003-0551-7
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DOI: https://doi.org/10.1007/s00402-003-0551-7