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Mixed layers of DPPC and a linear poly(ethylene glycol)-b-hyperbranched poly(glycerol) block copolymer having a cholesteryl end group

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Abstract

Interactions of the phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) with the amphiphilic diblock copolymer Ch-lPEG30-b-hbPG24 (ChP) are studied at the air–water interface by surface pressure–mean molecular area (π–mmA) measurements of mixed Langmuir films and adsorption measurements of ChP to the air–water interface covered with DPPC monolayers at different initial surface pressure values π 0. ChP is composed of a single hydrophobic cholesteryl (Ch) moiety covalently bound to a diblock copolymer consisting of a hydrophilic linear poly(ethylene glycol) (lPEG) block and a hydrophilic hyperbranched poly(glycerol) (hbPG) block. Langmuir isotherms and compression moduli of the mixed Langmuir films of different molar ratios reveal distinct interactions between DPPC and ChP during compression. It is demonstrated that the behavior of the DPPC/ChP mixtures is dominated by DPPC up to a molar ratio of 10:1, whereas the behavior is predominantly governed by ChP in mixtures with lower DPPC content (molar ratios of 5:1, 2:1, and 1:1). In adsorption measurements, a strong affinity of ChP to DPPC is observed after injection into the water subphase. The surface pressure value π in up to which ChP is able to penetrate into DPPC monolayers is determined to the remarkably high value of 48.2 mN/m which attests the favorable interactions between DPPC and the Ch moiety of ChP. Atomic force microscopy on LB films of DPPC/ChP mixtures of different molar ratios transferred onto hydrophilic substrates confirms the presence of two different phases, a DPPC-rich phase and a ChP-rich phase.

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Acknowledgments

J.K. thanks the Deutsche Forschungsgemeinschaft for funding (FOR 1145). A.M.H. is a recipient of a fellowship of the Graduate School of Excellence MAINZ (Materials Science in Mainz) through funding of the Excellence Initiative (DFG/GSC 266).

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Authors and Affiliations

  1. Department of Chemistry, Martin Luther University Halle-Wittenberg, 06099, Halle (Saale), Germany

    Xiaoju Peng, Sascha Reuter & Jörg Kressler

  2. Institute of Organic Chemistry, Johannes Gutenberg University, 55099, Mainz, Germany

    Anna Maria Hofmann & Holger Frey

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  1. Xiaoju Peng
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  2. Anna Maria Hofmann
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  3. Sascha Reuter
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  5. Jörg Kressler
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Correspondence to Jörg Kressler.

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Peng, X., Hofmann, A.M., Reuter, S. et al. Mixed layers of DPPC and a linear poly(ethylene glycol)-b-hyperbranched poly(glycerol) block copolymer having a cholesteryl end group. Colloid Polym Sci 290, 579–588 (2012). https://doi.org/10.1007/s00396-012-2613-2

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  • DOI: https://doi.org/10.1007/s00396-012-2613-2

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