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The effects of different high-fat (lard, soybean oil, corn oil or olive oil) diets supplemented with fructo-oligosaccharides on colonic alkaline phosphatase activity in rats

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Abstract

Purpose

We recently reported that fermentable non-digestible carbohydrates including fructo-oligosaccharides (FOS) commonly elevate colonic alkaline phosphatase (ALP) activity and the expression of IAP-I, an ALP gene, in rats fed a high-fat (HF) diet, and also elevate gut mucins and modulate gut microbiota. This study aims to investigate whether dietary fat types influence the effect of FOS on colonic ALP activity and the luminal environment in HF-fed rats.

Methods

Male Sprague–Dawley rats were fed a diet containing 30% soybean oil, corn oil, olive oil or lard with or without 4% FOS for 2 weeks. Colon ALP activity, gene expression, and gut luminal variables including mucins and microbiota were measured.

Results

In the lard diet groups, dietary FOS significantly elevated colonic ALP activity and the expression of IAP-I. The elevating effect of FOS on colonic ALP activity was also observed in the olive oil diet groups, although here the IAP-I expression was not changed. However, the soybean oil and corn oil diet groups did not exhibit the elevating effect of FOS on colon ALP. Fecal ALP and mucins were significantly elevated by dietary FOS regardless of dietary fat types, and the effect of FOS was prominent in the lard diet groups. The number of Lactobacillus spp. observed in fecal matter was significantly increased by dietary FOS in the lard and olive oil diet groups, but not in the soybean oil and corn oil diets groups.

Conclusion

This study suggests that dietary fat types may change the effect of FOS on the colonic luminal environment including the ALP activity in rats fed a high-fat diet.

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Abbreviations

ALP:

Alkaline phosphatase

FOS:

Fructo-oligosaccharides

HF:

High-fat

MUFA:

Monounsaturated fatty acid

PUFA:

Polyunsaturated fatty acids

SFA:

Saturated fatty acids

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Acknowledgements

This research was supported by JSPS KAKENHI Grant Number 18K02244.

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Authors and Affiliations

  1. Faculty of Human Life Sciences, Fuji Women’s University, Ishikari, 061-3204, Japan

    Yukako Okazaki

  2. Institution of Life Sciences and Nutrition, Sapporo, 001-0037, Japan

    Tetsuyuki Katayama

Authors
  1. Yukako Okazaki
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  2. Tetsuyuki Katayama
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Contributions

YO and TK conceived, designed and performed the experiments. YO analyzed the data. YO and TK wrote the paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yukako Okazaki.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Experimental procedures were reviewed and approved by the ethics committee for Animal experimentation of the Fuji Women’s University (approval no. 2018-2). All animal experiments were conducted according to the Guidelines for Animal experiments of the Fuji Women’s University, “Japanese Act on Welfare and Management of Animals” and “Standards Relating to the Care and Management of Laboratory Animals and Relief of Pain”. No adverse events were observed during this study.

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Okazaki, Y., Katayama, T. The effects of different high-fat (lard, soybean oil, corn oil or olive oil) diets supplemented with fructo-oligosaccharides on colonic alkaline phosphatase activity in rats. Eur J Nutr 60, 89–99 (2021). https://doi.org/10.1007/s00394-020-02219-y

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  • DOI: https://doi.org/10.1007/s00394-020-02219-y

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