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Hexane fraction from Laminaria japonica exerts anti-inflammatory effects on lipopolysaccharide-stimulated RAW 264.7 macrophages via inhibiting NF-kappaB pathway

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An Erratum to this article was published on 30 October 2012

Abstract

Purpose

Laminaria japonica is a representative marine brown alga used as a culinary item in East Asia. L. japonica extract was shown to exert various biological activities; however, its anti-inflammatory activity has not been reported. The aim of this study is to investigate the molecular mechanisms underlying its anti-inflammatory action.

Methods

Anti-inflammatory mechanisms of L. japonica n-hexane fraction (LHF) were assessed using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. An anti-inflammatory compound isolated from LHF by reverse-phase chromatography was identified using nuclear magnetic resonance (NMR) spectroscopy.

Results

Our results indicate that LHF significantly inhibited LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) secretion in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) with no cytotoxicity. As results, levels of pro-inflammatory cytokines were significantly reduced by pretreatment of LHF in LPS-stimulated RAW 264.7 cells. Treatment of LHF strongly suppressed nuclear factor-κB (NF-κB) promoter-driven expression and nuclear translocation of NF-κB by preventing proteolytic degradation of inhibitor of κB (IκB)-α in LPS-stimulated RAW 264.7 cells. Moreover, LHF inhibited the phosphorylation of Akt and mitogen-activated protein kinase (MAPK) in LPS-stimulated RAW 264.7 cells. One of the anti-inflammatory compounds was isolated from LHF and identified as fucoxanthin.

Conclusions

These results indicate that the LHF-mediated inhibition of NO and PGE2 secretion in LPS-stimulated macrophages is regulated by NF-κB inactivation through inhibition of IκB-α, MAPKs, and Akt phosphorylation. LHF may be considered as a functional food candidate for the prevention or treatment of inflammatory diseases.

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Acknowledgments

This work was financially supported by the Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and the program of the Brain Busan 21.

Conflict of interest

The authors declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Pukyong National University, 599-1, Daeyeon-dong, Nam-gu, Busan, 608-737, South Korea

    Ji-Young Lee, Min-Sup Lee, Hee-Jeon Choi, Ji-Woong Choi, Jae-Il Kim & Hyeung-Rak Kim

  2. Department of Food Science and Nutrition, Chonnam National University, Yeosu, 550-749, South Korea

    Taisun Shin

  3. Division of Applied Chemical Engineering, Pukyong National University, Busan, 608-739, South Korea

    Hee-Chul Woo

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  1. Ji-Young Lee
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Correspondence to Hyeung-Rak Kim.

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Lee, JY., Lee, MS., Choi, HJ. et al. Hexane fraction from Laminaria japonica exerts anti-inflammatory effects on lipopolysaccharide-stimulated RAW 264.7 macrophages via inhibiting NF-kappaB pathway. Eur J Nutr 52, 409–421 (2013). https://doi.org/10.1007/s00394-012-0345-1

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