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Colonoscopy as a tool for evaluating colorectal tumor development in a mouse model

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Purpose

A sporadic colon cancer mouse model with conditional mutations in adenomatous polyposis coli (Apc) is biologically relevant for human colorectal cancer (CRC). This study aimed to determine the utility and limitations of colonoscopy for evaluating colon tumors in this mouse model.

Methods

We compared the estimates of location, size, and miss rate of tumors detected during colonoscopy with those determined by necropsy. Sixty-six CPC-Apc mice originating from Apc F/wt mice harbor a Cdx2-Cre transgene in which colorectal tumorigenesis was driven by Apc allelic loss. The sensitivity and specificity of colonoscopy for detecting tumors in a mouse CRC model were investigated.

Results

A strong positive correlation was found between tumor location as measured by colonoscopy and the location determined by necropsy (p < 0.001). A total of 120 tumors were graded during colonoscopy (grades 1–5: 0, 8, 20, 27, and 65 lesions, respectively), and a strong positive correlation was found between the tumor grade determined by colonoscopy and size measured by necropsy (grades 2–5: 2.08, 2.98, 4.02, and 5.09 mm, respectively; p < 0.005). Although the miss rate was 47.1 %, most of the missed tumors (96 %) were in close proximity (within 5 mm) of another tumor.

Conclusions

A colonoscopic method for the reliable measurement of colorectal tumors in vivo has been established. The application of this technique to mouse models of colon carcinogenesis will provide a better understanding of the dynamics of tumor growth.

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Acknowledgments

We wish to thank the Nature Science Center for Basic Research and Development for this study and the Laboratory Animal Facility at Hiroshima University for maintaining the animals. Special thanks to Yuusuke Sotomaru, PhD and Tatsunari Sasada, MD for maintaining the animals and Minoru Hattori, PhD for statistical support.

Conflict of interest

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Authors and Affiliations

  1. Department of Surgery, Division of Frontier Medical Sciences, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

    Tomohiro Adachi & Yasuhumi Saito

  2. Department of Gastroenterological and Transplant Surgery Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, 7348551, Hiroshima, Japan

    Takao Hinoi, Masashi Miguchi, Manabu Shimomura & Hideki Ohdan

  3. Department of Gastroenterological and Transplant Surgery, Division of Medicine, Program of Medicine, Biomedical Sciences Major, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan

    Yuu Sasaki & Hiroaki Niitsu

Authors
  1. Tomohiro Adachi
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  2. Takao Hinoi
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  3. Yuu Sasaki
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  4. Hiroaki Niitsu
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  5. Yasuhumi Saito
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  6. Masashi Miguchi
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  7. Manabu Shimomura
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  8. Hideki Ohdan
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Corresponding author

Correspondence to Takao Hinoi.

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Adachi, T., Hinoi, T., Sasaki, Y. et al. Colonoscopy as a tool for evaluating colorectal tumor development in a mouse model. Int J Colorectal Dis 29, 217–223 (2014). https://doi.org/10.1007/s00384-013-1791-9

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  • DOI: https://doi.org/10.1007/s00384-013-1791-9

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