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Biodegradation of carbofuran by Pichia anomala strain HQ-C-01 and its application for bioremediation of contaminated soils

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Abstract

A novel yeast named HQ-C-01 was isolated from activated sludge and identified as Pichia anomala based on the morphology and 18S rDNA sequence analysis. The HQ-C-01 strain degraded 95.2% of carbofuran when the insecticide was used as the only C source and added at 50 mg/L in a mineral salts medium within 48 h. The optimal concentration, temperature, and pH of medium for degradation of carbofuran were 50 mg/L, 30°C, and pH 7.5, respectively. Strain HQ-C-01 could also effectively degrade other carbamate insecticides including carbaryl, indoxacarb, and fenobucarb, and the degradation rates were 99%, 85%, and 67%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain metabolized carbofuran to produce benzofuranol as the intermediate metabolite, which was further degraded. Degradation of carbofuran added at 50 mg/kg of soil was higher in yeast-inoculated soil than in the control. These results indicated that strain HQ-C-01 may potentially be used in bioremediation of carbofuran-contaminated soil.

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Acknowledgments

The researchers gratefully acknowledge the grants of National Natural Science Foundation of China (no. 30871660) and the Project of Scientific Technological Planning of Guangdong Province, China (no. 2009B020310005). The authors would like to thank Dr. Muhammad Rizwan-ul-haq for his helpful comments on the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Liu Yang, Shaohua Chen, Meiying Hu, Weining Hao, Peng Geng & Yanbo Zhang

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  1. Liu Yang
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  2. Shaohua Chen
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  3. Meiying Hu
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  4. Weining Hao
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  5. Peng Geng
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  6. Yanbo Zhang
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Correspondence to Meiying Hu.

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Yang, L., Chen, S., Hu, M. et al. Biodegradation of carbofuran by Pichia anomala strain HQ-C-01 and its application for bioremediation of contaminated soils. Biol Fertil Soils 47, 917–923 (2011). https://doi.org/10.1007/s00374-011-0602-0

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