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Comparative metabolomics analysis investigating the impact of melatonin-enriched diet on energy metabolism in the crayfish, Cherax destructor

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Abstract

Melatonin is a multifunctional bioactive molecule present in almost all organisms and has been gradually used in the aquaculture industry in recent years. Energy metabolism is an essential process for individuals to maintain their life activities; however, the process through which melatonin regulates energy metabolism in aquatic animals remains unclear. The present study aimed to conduct a comprehensive analysis of the regulatory mechanism of melatonin for energy metabolism in Cherax destructor by combining metabolomics analysis with the detection of the key substance content, enzymatic activity, and gene expression levels in the energy metabolism process after culturing with dietary melatonin supplementation for 8 weeks. Our results showed that dietary melatonin increased the content of glycogen, triglycerides, and free fatty acids; decreased lactate levels; and promoted the enzymatic activity of pyruvate kinase (PK), malate dehydrogenase (MDH), and acetyl-CoA carboxylase. The results of gene expression analysis showed that dietary melatonin also increased the expression levels of hexokinase, PK, MDH, lactate dehydrogenase, lipase, and fatty acid synthase genes. The results of metabolomics analysis showed that differentially expressed metabolites were significantly enriched in lysine degradation and glycerophospholipid metabolism. In conclusion, our study demonstrates that dietary melatonin increased oxidative phosphorylation, improved glucose utilization, and promoted storage of glycogen and lipids in C. destructor. These lipids are used not only for energy storage but also to maintain the structure and function of cell membranes. Our results further add to the understanding of the mechanisms of energy regulation by melatonin in crustaceans.

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Funding

This work was supported by the Project of Agriculture Research System of Shanghai, China (No. 202205). We thank International Science Editing (http://www.internationalscienceediting.com) for editing our manuscript.

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Authors and Affiliations

  1. School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China

    Ying Yang, Jiangtao Tian, Wenyue Xu, Cuobaima Ping, Xinglin Du, Yucong Ye, Bihong Zhu, Yizhou Huang & Yunlong Zhao

  2. Fishery Machinery and Instrument Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, 200092, China

    Yiming Li

  3. Freshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing, 210017, China

    Qichen Jiang

  4. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200241, China

    Yunlong Zhao

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  1. Ying Yang
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Contributions

YY: conceptualization, resources, formal analysis, investigation, visualization, writing—original draft, writing—review and editing. JT: conceptualization, formal analysis, investigation, writing—review and editing. WX: conceptualization, investigation, visualization, writing—review & editing. CP: formal analysis, Investigation, writing—review and editing. XD: conceptualization, formal analysis, writing—review and editing. YY: conceptualization, writing—review and editing. BZ: conceptualization, writing—review and editing. YH: conceptualization, writing—review and editing. YL: visualization, writing—review and editing. QJ: investigation, writing—review and editing. YZ: conceptualization, resources, formal analysis, visualization, writing—review and editing, project administration, funding acquisition.

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Correspondence to Yunlong Zhao.

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Yang, Y., Tian, J., Xu, W. et al. Comparative metabolomics analysis investigating the impact of melatonin-enriched diet on energy metabolism in the crayfish, Cherax destructor. J Comp Physiol B 193, 615–630 (2023). https://doi.org/10.1007/s00360-023-01518-0

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