Abstract
Melanocortin-4 receptor (Mc4r) function related to reproduction in fish has not been extensively investigated. Here, we report on gene expression changes by real-time PCR following treatment with Mc4r agonists and antagonists in the spotted scat (Scatophagus argus). Using in vitro incubated hypothalamus, the Mc4r nonselective agonist NDP-MSH ([Nle4, D-Phe7]-α-melanocyte stimulating hormone; 10−6 M) and selective agonist THIQ (N-[(3R)-1, 2, 3, 4-Tetrahydroisoquinolinium-3-ylcarbonyl]- (1R)-1-(4-chlorobenzyl)-2-[4-cyclohexyl-4-(1H-1,2,4-triazol-1-ylmethyl) piperidin-1-yl]-2-oxoethylamine; 10−7 M) significantly increased the expression of gnrh (Gonadotropin releasing hormone), while the Mc4r nonselective antagonist SHU9119 (Ac-Nle-[Asp-His-DPhe/DNal(2′)-Arg-Trp-Lys]-NH2; 10−6 M) and selective antagonist Ipsen 5i (compound 5i synthesized in Ipsen Research Laboratories; 10−6 M) significantly inhibited gnrh expression after 3 h of incubation. In incubated pituitary tissue, NDP-MSH and THIQ significantly increased the expression of fshb (Follicle-stimulating hormone beta subunit) and lhb (Luteinizing hormone beta subunit), while SHU9119 and Ipsen 5i significantly decreased fshb and lhb expression after 3 h of incubation. During the in vivo experiment, THIQ (1 mg/kg bw) significantly increased gnrh expression in hypothalamic tissue, as well as the fshb and lhb expression in pituitary tissue 12 h after abdominal injection. Furthermore, Ipsen 5i (1 mg/kg bw) significantly inhibited gnrh expression in hypothalamic tissue, as well as fshb and lhb gene expression in pituitary tissue 12 h after abdominal injection. In summary, Mc4r singling appears to stimulate gnrh expression in the hypothalamus, thereby modulating the synthesis of Fsh and Lh in the pituitary. In addition, Mc4r also appears to directly regulate fshb and lhb levels in the pituitary in spotted scat. Our study suggests that Mc4r, through the hypothalamus and pituitary, participates in reproductive regulation in fish.
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Abbreviations
- ACTH:
-
Adrenocorticotropic hormone
- AGRP:
-
Agouti-related protein
- cAMP:
-
Cyclic adenosine monophosphate
- Fsh:
-
Follicle-stimulating hormone
- GtH:
-
Gonadotropin hormones
- HPG:
-
Hypothalamic–pituitary–gonadal
- HPPS:
-
Hypothalamo-pituitary portal system
- Ipsen 5i:
-
Compound 5i synthesized in Ipsen Research Laboratories
- Lh:
-
Luteinizing hormone
- MCR:
-
Melanocortin receptor
- Mc4r:
-
Melanocortin-4 receptor
- α-MSH:
-
α-melanocyte-stimulating hormone
- NDP-MSH:
-
[Nle4, D-Phe7]-α-melanocyte-stimulating hormone
- POMC:
-
Proopiomelanocortin
- Gnrh:
-
Gonadotropin-releasing hormone
- SHU9119:
-
(Ac-Nle-[Asp-His-DPhe/DNal(2′)-Arg-Trp-Lys]-NH2)
- THIQ:
-
(N-[(3R)-1,2,3,4-tetrahydroisoquinolinium3-ylcarbonyl]-(1R)-1-(4-chlorobenzyl)-2-[4-cyclohexyl-4-(1 H-1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-2-oxoethylamine)
References
Aspiras AC, Rohner N, Martineau B, Borowsky RL, Tabin CJ (2015) Melanocortin 4 receptor mutations contribute to the adaptation of cavefish to nutrient-poor conditions. Proc Natl Acad Sci USA 112(31):9668–9673
Caruso C, Carniglia L, Durand D, Gonzalez PV, Scimonelli TN, Lasaga M (2012) Melanocortin 4 receptor activation induces brain-derived neurotrophic factor expression in rat astrocytes through cyclic AMP-protein kinase A pathway. Mol Cell Endocrinol 348(1):47–54.
Cerdá-Reverter JM, Ringholm A, Schiöth HB, Peter RE (2003a) Molecular cloning, pharmacological characterization, and brain mapping of the melanocortin 4 receptor in the goldfish: involvement in the control of food intake. Endocrinology 144(6):2336–2349
Cerdá-Reverter JM, Schiöth HB, Peter RE (2003b) The central melanocortin system regulates food intake in goldfish. Regul Pept 115(2):101–113
Chai B, Li JY, Zhang W, Newman E, Ammori J, Mulholland MW (2006) Melanocortin-4 receptor-mediated inhibition of apoptosis in immortalized hypothalamic neurons via mitogen-activated protein kinase. Peptides 27(11):2846–2857
Chen HP, Deng SP, Dai ML, Zhu CH, Li GL (2016) Molecular cloning, characterization, and expression profiles of androgen receptors in spotted scat (Scatophagus argus). Genet Mol Res 15(2). doi:10.4238/gmr.15027838
Cone RD (2006) Studies on the physiological functions of the melanocortin system. Endocr Rev 27(7):736–749
Dores RM, Lecaude S (2005) Trends in the evolution of the proopiomelanocortin gene. Gen Comp Endocrinol 142(1–2):81–93
Dufour S, Sebert ME, Weltzien FA, Rousseau K, Pasqualini C (2010) Neuroendocrine control by dopamine of teleost reproduction. J Fish Biol 76(1):129–160
Farooqi IS, Keogh JM, Yeo GS, Lank EJ, Cheetham T, O’Rahilly S (2003) Clinical spectrum of obesity and mutations in the melanocortin 4 receptor gene. N Engl J Med 348(12):1085–1095
Graham M, Shutter JR, Sarmiento U, Sarosi I, Stark KL (1997) Overexpression of Agrt leads to obesity in transgenic mice. Nat Genet 17(3):273–274
Grieco P, Cai M, Han G, Trivedi D, Campiglia P, Novellino E, Hruby VJ (2007) Further structure-activity studies of lactam derivatives of MT-II and SHU-9119: their activity and selectivity at human melanocortin receptors 3, 4, and 5. Peptides 28(6):1191–1196
Huszar D, Lynch CA, Fairchild-Huntress V, Dunmore JH, Fang Q, Berkemeier LR, Gu W, Kesterson RA, Boston BA, Cone RD, Smith FJ, Campfield LA, Burn P, Lee F (1997) Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell 88(1):131–141
Ilnytska O, Argyropoulos G (2008) The role of the Agouti-related protein in energy balance regulation. Cell Mol Life Sci 65(17):2721–2731
Jadhao AG, Pinelli C, D’Aniello B, Tsutsui K (2016) Gonadotropin-inhibitory hormone (GnIH) in the amphibian brain and its relationship with the gonadotropin releasing hormone (GnRH) system: An overview. Gen Comp Endocrinol 240:69–76
Jangprai A, Boonanuntanasarn S, Yoshizaki G (2011) Characterization of melanocortin 4 receptor in Snakeskin Gourami and its expression in relation to daily feed intake and short-term fasting. Gen Comp Endocrinol 173(1):27–37
Khong K, Kurtz SE, Sykes RL, Cone RD (2001) Expression of functional melanocortin-4 receptor in the hypothalamic GT1-1 cell line. Neuroendocrinology 74(3):193–201
Klebig ML, Wilkinson JE, Geisler JG, Woychik RP (1995) Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur. Proc Natl Acad Sci USA 92(11):4728–4732
Klovins J, Haitina T, Fridmanis D, Kilianova Z, Kapa I, Fredriksson R, Gallo-Payet N, Schiöth HB (2004) The melanocortin system in Fugu: determination of POMC/AGRP/MCR gene repertoire and synteny, as well as pharmacology and anatomical distribution of the MCRs. Mol Biol Evol 21(3):563–579
Kobayashi Y, Tsuchiya K, Yamanome T, Schiöth HB, Kawauchi H, Takahashi A (2008) Food deprivation increases the expression of melanocortin-4 receptor in the liver of barfin flounder, Verasper moseri. Gen Comp Endocrinol 155(2):280–287
Lampert KP, Schmidt C, Fischer P, Volff JN, Hoffmann C, Muck J, Lohse MJ, Ryan MJ, Schartl M (2010) Determination of onset of sexual maturation and mating behavior by melanocortin receptor 4 polymorphisms. Curr Biol 20(19):1729–1734
Langouche L, Hersmus N, Papageorgiou A, Vankelecom H, Denef C (2004) Melanocortin peptides stimulate prolactin gene expression and prolactin accumulation in rat pituitary aggregate cell cultures. J Neuroendocrinol 16(8):695–703
Li GL, Zhang MZ, Deng SP, Chen HP, Zhu CH (2015) Effects of temperature and fish oil supplementation on ovarian development and foxl2 mRNA expression in spotted scat Scatophagus argus. J Fish Biol 86(1):248–260
Li JT, Yang Z, Chen HP, Zhu CH, Deng SP, Li GL, Tao YX (2016) Molecular cloning, tissue distribution, and pharmacological characterization of melanocortin-4 receptor in spotted scat, Scatophagus argus. Gen Comp Endocrinol 230–231:143–152
Limone P, Calvelli P, Altare F, Ajmone-Catt P, Lima T, Molinatti GM (1997) Evidence for an interaction between alpha-MSH and opioids in the regulation of gonadotropin secretion in man. J Endocrinol Invest 20(4):207–210
Martin WJ, McGowan E, Cashen DE, Gantert LT, Drisko JE, Hom GJ, Nargund R, Sebhat I, Howard AD, Van der Ploeg LH, MacIntyre DE (2002) Activation of melanocortin MC(4) receptors increases erectile activity in rats ex copula. Eur J Pharmacol 454(1):71–79
Mountjoy KG, Robbins LS, Mortrud MT, Cone RD (1992) The cloning of a family of genes that encode the melanocortin receptors. Science 257(5074):1248–1251
Mountjoy KG, Mortrud MT, Low MJ, Simerly RB, Cone RD (1994) Localization of the melanocortin-4 receptor (MC4-R) in neuroendocrine and autonomic control circuits in the brain. Mol Endocrinol 8(10):1298–1308
Mountjoy KG, Jenny Wu CS, Dumont LM, Wild JM (2003) Melanocortin-4 receptor messenger ribonucleic acid expression in rat cardiorespiratory, musculoskeletal, and integumentary systems. Endocrinology 144(12):5488–5496
Nordheim U, Nicholson JR, Dokladny K, Dunant P, Hofbauer KG (2006) Cardiovascular responses to melanocortin 4-receptor stimulation in conscious unrestrained normotensive rats. Peptides 27(2):438–443
Ollmann MM, Wilson BD, Yang YK, Kerns JA, Chen Y, Gantz I, Barsh GS (1997) Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein. Science 278(5335):135–138
Peng C, Chang JP, Yu KL, Wong AO, Van Goor F, Peter RE, Rivier JE (1993) Neuropeptide-Y stimulates growth hormone and gonadotropin-II secretion in the goldfish pituitary: involvement of both presynaptic and pituitary cell actions. Endocrinology 132(4):1820–1829
Poitout L, Brault V, Sackur C, Bernetière S, Camara J, Plas P, Roubert P (2007) Identification of a novel series of benzimidazoles as potent and selective antagonists of the human melanocortin-4 receptor. Bioorg Med Chem Lett 17(16):4464–4470
Rebers FE, Bosma PT, van Dijk W, Goos HJ, Schulz RW (2000) GnRH stimulates LH release directly via inositol phosphate and indirectly via cAMP in African catfish. Am J Physiol Regul Integr Comput Physiol 278(6):R1572–R1578
Ringholm A, Fredriksson R, Poliakova N, Yan YL, Postlethwait JH, Larhammar D, Schiöth HB (2002) One melanocortin 4 and two melanocortin 5 receptors from zebrafish show remarkable conservation in structure and pharmacology. J Neurochem 82(1):6–18
Ringholm A, Klovins J, Fredriksson R, Poliakova N, Larson ET, Kukkonen JP, Larhammar D, Schiöth HB (2003) Presence of melanocortin (MC4) receptor in spiny dogfish suggests an ancient vertebrate origin of central melanocortin system. Eur J Biochem 270(2):213–221
Sánchez E, Rubio VC, Thompson D, Metz J, Flik G, Millhauser GL, Cerdá-Reverter JM (2009) Phosphodiesterase inhibitor-dependent inverse agonism of agouti-related protein on melanocortin 4 receptor in sea bass (Dicentrarchus labrax). Am J Physiol Regul Integr Comp Physiol 296(5):R1293–R1306
Sandrock M, Schulz A, Merkwitz C, Schöneberg T, Spanel-Borowski K, Ricken A (2009) Reduction in corpora lutea number in obese melanocortin-4-receptor-deficient mice. Reprod Biol Endocrinol 7:24
Sebag JA, Zhang C, Hinkle PM, Bradshaw AM, Cone RD (2013) Developmental control of the melanocortin-4 receptor by MRAP2 proteins in zebrafish. Science 341(6143):278–281
Tao YX (2010) The melanocortin-4 receptor: physiology, pharmacology, and pathophysiology. Endocr Rev 31(4):506–543
Tao YX, Huang H (2014) Ipsen 5i is a novel potent pharmacoperone for intracellularly retained melanocortin-4 receptor mutants. Front Endocrinol (Lausanne) 5:131
Thompson IR, Ciccone NA, Xu S, Zaytseva S, Carroll RS, Kaiser UB (2013) GnRH pulse frequency-dependent stimulation of FSHβ transcription is mediated via activation of PKA and CREB. Mol Endocrinol 27(4):606–618
Trudeau VL, Spanswick D, Fraser EJ, Larivière K, Crump D, Chiu S, MacMillan M, Schulz RW (2000) The role of amino acid neurotransmitters in the regulation of pituitary gonadotropin release in fish. Biochem Cell Biol 78(3):241–259
Tsutsui K, Ubuka T, Bentley GE, Kriegsfeld LJ (2012) Gonadotropin-inhibitory hormone (GnIH): discovery, progress and prospect. Gen Comp Endocrinol 177(3):305–314
Vadakkadath MS, Atwood CS (2005) The role of hypothalamic–pituitary–gonadal hormones in the normal structure and functioning of the brain. Cell Mol Life Sci 62(3):257–270
Van der Ploeg LH, Martin WJ, Howard AD, Nargund RP, Austin CP, Guan X, Drisko J, Cashen D, Sebhat I, Patchett AA, Figueroa DJ, DiLella AG, Connolly BM, Weinberg DH, Tan CP, Palyha OC, Pong SS, MacNeil T, Rosenblum C, Vongs A, Tang R, Yu H, Sailer AW, Fong TM, Huang C, Tota MR, Chang RS, Stearns R, Tamvakopoulos C, Christ G, Drazen DL, Spar BD, Nelson RJ, MacIntyre DE (2002) A role for the melanocortin 4 receptor in sexual function. Proc Natl Acad Sci USA 99(17):11381–11386
Vulliémoz NR, Xiao E, Xia-Zhang L, Wardlaw SL, Ferin M (2005) Central infusion of agouti-related peptide suppresses pulsatile luteinizing hormone release in the ovariectomized rhesus monkey. Endocrinology 146(2):784–789
Ward DR, Dear FM, Ward IA, Anderson SI, Spergel DJ, Smith PA, Ebling FJ (2009) Innervation of gonadotropin-releasing hormone neurons by peptidergic neurons conveying circadian or energy balance information in the mouse. PLoS One 4(4):e5322
Watanobe H, Schiöth HB, Wikberg JE, Suda T (1999) The melanocortin 4 receptor mediates leptin stimulation of luteinizing hormone and prolactin surges in steroid-primed ovariectomized rats. Biochem Biophys Res Commun 257(3):860–864
Yaron Z, Gur G, Melamed P, Rosenfeld H, Levavi-Sivan B, Elizur A (2001) Regulation of gonadotropin subunit genes in tilapia. Comp Biochem Physiol B Biochem Mol Biol 129(2–3):489–502
Yaron Z, Gur G, Melamed P, Rosenfeld H, Elizur A, Levavi-Sivan B (2003) Regulation of fish gonadotropins. Int Rev Cytol 225:131–185
Yeo GS, Farooqi IS, Aminian S, Halsall DJ, Stanhope RG, O’Rahilly S (1998) A frameshift mutation in MC4R associated with dominantly inherited human obesity. Nat Genet 20(2):111–112
Yeo GS, Farooqi IS, Challis BG, Jackson RS, O’Rahilly S (2000) The role of melanocortin signalling in the control of body weight: evidence from human and murine genetic models. QJM 93(1):7–14
Zhang Z, Lau SW, Zhang L, Ge W (2015) Disruption of zebrafish follicle-stimulating hormone receptor (Fshr) but not luteinizing hormone receptor (lhcgr) gene by talen leads to failed follicle activation in females followed by sexual reversal to males. Endocrinology 156(10):3747–3762
Zohar Y, Muñoz-Cueto JA, Elizur A, Kah O (2010) Neuroendocrinology of reproduction in teleost fish. Gen Comp Endocrinol 165(3):438–455
Acknowledgements
This study was supported by grants from Zhanjiang Science and Technology Bureau (2016A03017), Natural Science Foundation of Guangdong Province (2016A030313743), Guangdong Ocean University Natural Science Research Program (2015 and 2016), Project of Provincial Key Platform and Major Scientific Research of Colleges and Universities in Guangdong (2015KTSCX058), Sail Projects of Guangdong (2014.1), Marine Fishery Science and Technology Extension Projects of Guangdong (A201408A06 and A201608B01).
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Communicated by H.V. Carey.
D.-N. Jiang and J.-T. Li contributed equally and should be considered as co-first authors.
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Jiang, DN., Li, JT., Tao, YX. et al. Effects of melanocortin-4 receptor agonists and antagonists on expression of genes related to reproduction in spotted scat, Scatophagus argus . J Comp Physiol B 187, 603–612 (2017). https://doi.org/10.1007/s00360-017-1062-0
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DOI: https://doi.org/10.1007/s00360-017-1062-0