Abstract
A stereoscopic micro-PIV (stereo-μPIV) system for the simultaneous measurement of all three components of the velocity vector in a measurement plane (2D–3C) in a closed microchannel has been developed and first test measurements were performed on the 3D laminar flow in a T-shaped micromixer. Stereomicroscopy is used to capture PIV images of the flow in a microchannel from two different angles. Stereoscopic viewing is achieved by the use of a large diameter stereo objective lens with two off-axis beam paths. Additional floating lenses in the beam paths in the microscope body allow a magnification up to 23×. The stereo-PIV images are captured simultaneously by two CCD cameras. Due to the very small confinement, a standard calibration procedure for the stereoscopic imaging by means of a calibration target is not feasible, and therefore stereo-μPIV measurements in closed microchannels require a calibration based on the self-calibration of the tracer particle images. In order to include the effects of different refractive indices (of the fluid in the microchannel, the entrance window and the surrounding air) a three-media-model is included in the triangulation procedure of the self-calibration. Test measurement in both an aligned and a tilted channel serve as an accuracy assessment of the proposed method. This shows that the stereo-μPIV results have an RMS error of less than 10% of the expected value of the in-plane velocity component. First measurements in the mixing region of a T-shaped micromixer at Re = 120 show that 3D flow in a microchannel with dimensions of 800 × 200 μm2 can be measured with a spatial resolution of 44 × 44 × 15 μm3. The stationary flow in the 200 μm deep channel was scanned in multiple planes at 22 μm separation, providing a full 3D measurement of the averaged velocity distribution in the mixing region of the T-mixer. A limitation is that this approach requires a stereo-objective that typically has a low NA (0.14–0.28) and large depth-of-focus as opposed to high NA lenses (up to 0.95 without immersion) for standard μPIV.
Similar content being viewed by others
References
Bourdon CJ, Olsen MG, Gorby AD (2004) Validation of an analytical solution for depth of correlation in microscopic particle image velocimetry. Meas Sci Technol 15:318–327
Bown MR, MacInnes JM, Allen RWK, Zimmermann WBJ (2005) Three-component micron resolution velocity measurements using sterescopic Micro-PIV. In: Proceedings of the 6th international symposium on particle image velocimentry, paper S06–S06
Delnoij E, Westerweel J, Deen NG, Kuipers JAM, van Swaaij WPM (1999) Ensemble correlation PIV applied to bubble plumes rising in a bubble column. Chem Eng Sci 54:5159–5171
Engler M, Kockmann N, Kiefer T, Woias P (2004) Numerical and experimental investigations on liquid mixing in static micromixers. Chem Eng J 101:315–322
Hoffmann M, Schlüter M, Räbiger N (2006) Experimental investigation of liquid–liquid mixing in T-shaped micro-mixers using μ-LIF and μ-PIV. Chem Eng Sci 61:2968–2976
Ikeda K, Ooms T, Westerweel J (2005) Feasibility study on microscopic digital holographic PIV. In: Proceedings of the PIVNET2 international workshop on μPIV and applications in microsystems, Delft
Keane RD Adrian RJ (1990) Optimization of particle image velocimeters. Part I: double pulsed systems. Meas Sci Technol 1:1202–1215
Klank H, Goranovic G, Kutter JP, Gjelstrup H, Michelsen J, Westergaard CH (2003) PIV measurements in a microfluidic 3D-sheathing structure with three-dimensional flow behaviour. J Micromech Microeng 12:862–869
Klasen LG, Brede M, Leder A (2005) Design and development of a stereo micro PIV—system. In: Proceedings of the PIVNET2 international workshop on μPIV and applications in microsystems, Delft
Kockmann N, Engler M, Haller D, Woias P (2005) Fluid dynamics and transfer processes in bended microchannels. Heat Transf Eng 26:71–78
Lammerding J, Rohály J, Hart DP (2002) Monocular 3-D magnetic bead microrheometry. In: Abstracts of the 11th international symposium application of laser techniques to fluid mechanics, paper 1–1, Lisbon
Lawson NJ, Wu J (1997) Three-dimensional particle image velocimetry: error analysis of stereoscopic techniques. Meas Sci Technol 8:894–900
Li H, Sadr R, Yoda M (2006) Multilayer Nano-particle image velocimetry. Exp Fluids (in press)
Lindken R, Westerweel J, Wieneke B (2005a) Stereoscopic micro PIV applied to the three-dimensional flow in a T-mixer. In: Proceedings of the PIVNET2 international workshop on μPIV and applications in microsystems, Delft
Lindken R, Westerweel J, Wieneke B (2005b) Development of a stereoscopic micro particle image velocimetry system (stereo-μPIV) and first measurements of the three-dimensional flow structure in a T-micromixer.In: Proceedings of the euromech colloquium 472 on microfluidics and transfer, Grenoble
Maas HG (1992) Contributions of digital photogrammetry to 3D PTV. In: Dracos T (ed) Three-dimensional velocity and vorticity. Measuring and image analysis techniques. Kluwer, Dordrecht, pp 191–208
Meinhart CD, Wereley ST, Santiago JG (1999) PIV measurements of a microchannel flow. Exp Fluids 27:414–419
Meinhart CD, Wereley ST, Gray HB (2000a) Volume illumination for two-dimensional particle image velocimetry. Meas Sci Technol 11:809–814
Meinhart CD, Wereley ST, Santiago JG (2000b) A PIV algorithm for estimating time-averaged velocity fields. J Fluids Eng 122:285–289
Meinhart CD, Wereley ST (2003) The theory of diffraction-limited resolution in microparticle image velocimetry. Meas Sci Technol 14:1047–1053
Olsen MG, Adrian RJ (2000) Out-of-focus effects on particle image visibility and correlation in microscopic particle image velocimetry. Exp Fluids 29:166–174
Park JS, Choi CK, Kihm KD (2004a) Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM). Exp Fluids 37:105–119
Park JS, Choi CK, Kihm KD (2004b) Nanoparticle tracking using CLSM (confocal laser scanning microscopy) and OSSM (optical serial sectioning microscopy) imaging. J Heat Transf 126:504
Park JS, Choi CK, Kihm KD (2005) Temperature measurement for nanoparticle (500-nm) suspension by detecting the Brownian motion using optical serial sectioning microscopy (OSSM). Meas Sci Technol 16:1418–1429
Park JS, Choi CK, Kihm KD (2006) Three-dimensional micro-PTV using deconvolution microscopy. Exp Fluids 40:491–499
Prasad AK, Adrian RJ (1993) Stereoscopic particle image velocimetry applied to liquid flows. Exp Fluids 15:49–60
Prasad AK (2000) Stereoscopic particle image velocimetry. Exp Fluids 29:103–116
Rohály J, Lammerding J, Frigerio F, Hart DP (2001) Monocular 3-D active micro-PTV. In: Proceedings of the 4th international symposium on particle image velocimetry, paper 1147
Santiago JG, Wereley ST, Meinhart CD, Beebe DJ, Adrian RJ (1998) A particle image velocimetry system for microfluidics. Exp Fluids 25:316–319
Schlüter M, Hoffmann M, Räbiger N (2005) Investigations on micromixing in T-shaped micromixers using μ-LIF and μ-PIV. In: Proceedings of the PIVNET2 international workshop on μPIV and applications in microsystems
Schneckenburger H, Stock K, Lyttek M, Strauss WSL, Sailer R (2003) Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells. Photochem Photobiol Sci 3:127–131
Speidel M, Jonas A, Florin EL (2003) Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging. Opt Lett 28:69–71
Westerweel J (1997) Fundamentals of digital particle image velocimetry. Meas Sci Technol 8:1379–1392
Westerweel J (2000) Theoretical analysis of the measurement precision in particle image velocimetry. Exp Fluids 29:S3–12
White FM (1974) Viscous fluid flow. McGraw-Hill, New York. ISBN 0-07-069710-8
Wieneke B (2005) Stereo-PIV using self-calibration on particle images. Exp Fluids 39:267–280
Willert CE, Gharib M (1992) 3-dimensional particle imaging with a single camera. Exp Fluids 12:353–358
Wong SH, Ward MCL, Wharton CW (2004) Micro T-mixer as a rapid mixing micromixer. Sensors Actuators B 100:359–379
Wu M, Roberts JW, Buckley M (2005) Three-dimensional fluorescent particle tracking at micron-scale using a single camera. Exp Fluids 38:461–465
Yang CT, Chuang HS (2005) Measurement of a microchamber flow by using a hybrid multiplexing holographic velocimetry. Exp Fluids 39:385–396
Yoda M (2005) Nano-particle image velocity (nPIV) Measurements inside the diffuse electric double layer in electroosmotic flow. In: Proceedings of the PIVNET2 international workshop on μPIV and applications in microsystems, Delft
Yoon SY, Kim KC (2005) Three dimensional particle tracking and velocity measurement in a microchannel by using an aperture with three holes. Proceedings of the 6th international symposium on particle image velocimentry, paper S10–06
Acknowledgement
The authors would like to thank Cor Rops (TNO Science & Industry, Delft, The Netherlands) for the help with the production of the microchannels and Peter Vennemann (Aero- and Hydrodynamics, TU Delft, The Netherlands) for the help with setting up the stereoscopic microscope for the measurements.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lindken, R., Westerweel, J. & Wieneke, B. Stereoscopic micro particle image velocimetry. Exp Fluids 41, 161–171 (2006). https://doi.org/10.1007/s00348-006-0154-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00348-006-0154-5