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Curcumin protects against ischemia/reperfusion injury in rat kidneys

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Abstract

Objectives

Renal ischemia followed by reperfusion leads to acute renal failure in both native kidneys and renal allograft. We investigated the effect of curcumin on ischemia-reperfusion (I/R) injury and the antioxidant effects of curcumin in rats.

Methods

Thirty rats were randomly divided into five experimental groups (control, sham, curcumin, I/R and I/R + curcumin, n = 6 each). Curcumin was administered (200 mg kg−1) orally to curcumin and I/R + curcumin groups for 7 days. Then, the rats were subjected to bilateral renal ischemia for 45 min and followed by reperfusion for 24 h. All rats were killed and kidney function tests, serum and tissue nitric oxide (NO), protein carbonyl (PC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined. Histopathological examinations were also performed.

Results

Curcumin significantly improved the urea and cystatin C levels in I/R + curcumin group compared to I/R group (p < 0.05). Reduction of serum GSH-Px was significantly improved by curcumin (p < 0.001), but SOD enzyme activity did not alter (p > 0.05). Treatment with curcumin also resulted in significant reduction in serum and tissue MDA, NO and PC and for tissue that were increased by renal I/R injury (p < 0.001 for serum and p < 0.05 for tissue, respectively). In histological examination, the rats treated with curcumin had nearly normal morphology of the kidney.

Conclusions

Based on our results, it can be concluded that curcumin protects the kidneys against I/R injury via its antioxidant effects.

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Conflict of interest statement

There is no conflict of interest.

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Authors and Affiliations

  1. Department of Urology, Fatih University, School of Medicine, Ankara, Turkey

    Omer Bayrak, Mehmet Erol Yıldırım & Ersin Cimentepe

  2. Department of Nephrology, Fatih University, School of Medicine, Hosdere cad No: 145, Y. Ayranci, 06540, Ankara, Turkey

    Ebru Uz, Faruk Turgut & Ali Akcay

  3. Department of Pathology, Fatih University, School of Medicine, Ankara, Turkey

    Reyhan Bayrak

  4. Department of Urology, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

    Ali Fuat Atmaca

  5. Department of Biochemistry, Gaziosmanpasa University, Tokat, Turkey

    Semsettin Sahin

  6. Department of Internal Medicine, Fatih University, School of Medicine, Ankara, Turkey

    Arif Kaya

Authors
  1. Omer Bayrak
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  2. Ebru Uz
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  3. Reyhan Bayrak
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  4. Faruk Turgut
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  7. Mehmet Erol Yıldırım
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  8. Arif Kaya
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  9. Ersin Cimentepe
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Corresponding author

Correspondence to Faruk Turgut.

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Bayrak, O., Uz, E., Bayrak, R. et al. Curcumin protects against ischemia/reperfusion injury in rat kidneys. World J Urol 26, 285–291 (2008). https://doi.org/10.1007/s00345-008-0253-4

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  • DOI: https://doi.org/10.1007/s00345-008-0253-4

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