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MicroRNA775 Targets a Probable β-(1,3)-Galactosyltransferase to Regulate Growth and Development in Arabidopsis thaliana

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Abstract

MicroRNAs are critical regulators of gene expression in plants and other organisms, and are involved in regulating plethora of developmental processes. Evolutionarily, miRNAs can be ancient and conserved across species or recently evolved and young, which are not conserved across diverse plant groups. MicroRNA775 (miR775) is a non-conserved miRNA identified only in Arabidopsis thaliana (A. thaliana). Here, we investigated the functional significance of miR775 in A. thaliana and observed that miR775 targets a probable β-(1,3)-galactosyltransferase gene at post transcriptional level. Phenotypic analysis of miR775 over-expression lines and the target mutant suggested miR775 regulates rosette size by elongating petiole length and increasing leaf area. Further, the expression of miR775 was found to be up-regulated in response to UV-B and hypoxia. Our results also suggest that miR775 regulated β-(1,3)-galactosyltransferase may involve in regulating the β-(1,3)-galactan content of arabinogalactans. Collectively, our findings establish a role of miR775 in regulating growth and development in A. thaliana.

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Acknowledgements

The authors acknowledge the financial support of Council of Scientific and Industrial Research (CSIR), New Delhi and partly by Department of Biotechnology (DBT), Government of India, New Delhi, India. PM and AKV also acknowledge the University Grant Commission (UGC), New Delhi for providing the fellowship.

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Authors and Affiliations

  1. Molecular Biology and Biotechnology Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Parneeta Mishra, Akanksha Singh, Ashwani Kumar Verma, Rajneesh Singh & Sribash Roy

  2. Microbial Technologies Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Shashank Kumar Mishra

  3. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Parneeta Mishra, Akanksha Singh, Ashwani Kumar Verma & Sribash Roy

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  1. Parneeta Mishra
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  2. Akanksha Singh
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  4. Shashank Kumar Mishra
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  5. Rajneesh Singh
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Contributions

PM, AS, AKV and RS conducted the experiments. SKM conducted GC–MS analysis. PM analysed the results and wrote the manuscript with SR. All authors read and approved the manuscript. SR designed and arranged fund for the experiments.

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Correspondence to Sribash Roy.

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Mishra, P., Singh, A., Verma, A.K. et al. MicroRNA775 Targets a Probable β-(1,3)-Galactosyltransferase to Regulate Growth and Development in Arabidopsis thaliana. J Plant Growth Regul 41, 3271–3284 (2022). https://doi.org/10.1007/s00344-021-10511-2

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