Abstract
Qinghai-Tibet plateau as an alpine and high-altitude area is frequently confronted with freeze-thaw stress. Recently, due to the change of global climate, the drought and salinization of soil in this area have become increasingly serious, together with low temperature, and constitute the main limiting factors for crop growth in this area. Physiological responses of blue barley to salinization (S), drought (D), and freeze-thaw (F) stress were studied. Through applying 100 mmol/L NaCl, 15% drought and freeze-thaw cycle (10, − 5, 10 °C) stress, the changes of relative water content (RWC), soluble protein, malondialdehyde (MDA), superoxide dismutase (SOD), and peroxidase (POD) in seedling leaves of 8 treatment groups (CK, S, D, DS, F, FD, FS, and FDS) were analyzed. The results showed that single stress, either NaCl, drought, or freeze-thaw, could lead to the decrease of RWC and the increase of not only the soluble protein and MDA content, but also the SOD and POD activity in leaves. During the freeze-thaw cycle, the soluble protein, MDA content, SOD, and POD activity of seedlings increased at first and gradually decreased, while RWC gradually decreased with the change of temperature. The research indicated that there were synergistic effects among the stresses of drought, NaCl, and freeze-thaw on the plant, and the compound stress could cause more serious effect on the plant compared with the single one.
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Bao G, Ao Q, Li Q, Bao Y, Zheng Y, Feng X, Ding X (2017) Physiological characteristics of medicago sativa L. In response to acid deposition and freeze-thaw stress. Water Air Soil Pollut 228:376
Bao G, Zhang M, Li Y, Chang Y, Tang W, Zhu S, Fan C, Ding X (2019) Physiological responses of alfalfa seedlings to freeze-thaw cycles and alkaline salt stress. Fresenius Environ Bull 28:4114–4122
Bian W, Bao G, Qian H, Song Z, Qi Z, Zhang M, Chen W, Dong W (2018) Physiological response characteristics in Medicago sativa under freeze-thaw and deicing salt stress. Water Air Soil Pollut 229:196
Chen K, Renaut J, Sergeant K, Wei H, Arora R (2013) Proteomic changes associated with freeze-thaw injury and post-thaw recovery in onion (Allium cepa l.) scales. Plant Cell Environ 36:892–905
Ding Y, Jing P, Liu P (2012) Advances in research on gene expression, activity regulation and stress resistance of plant sod. Biol Teach 37:6–8
Duan R, Ren Y, Xiong H (2009) Effects of low temperature stress on chill-resistance physiological indexes of highland barley seedlings. Anhui Agric Sci 37:16796–16797
Duan H, Zhao T, Fu W, Zeng X, Xie X, Yi F (2012) Effects of nacl stress on some physiological indices in hulless barley seedlings. J Neijiang Teach Coll 27:42–44
Farooq M, Kobayashi N, Ito O, Wahid A, Serraj R (2010) Broader leaves result in better performance of indica rice under drought stress. J Plant Physiol 167:1066–1075
Feng B, Yu H, Hu Y, Gao X, Gao J, Gao D, Zhang S (2009) The physiological characteristics of the low canopy temperature wheat (Triticum aestivum l.) genotypes under simulated drought condition. Acta Physiol Plant 31:1229–1235
Fischer S, Wilckens R, Jara J, Aranda M (2013) Variation in antioxidant capacity of quinoa (Chenopodium quinoa will) subjected to drought stress. Ind Crops Prod 46:341–349
França MGC, Thi ATP, Pimentel C, Rossiello ROP, Zuily-Fodil Y, Laffray D (2019) Differences in growth and water relations among phaseolus vulgaris cultivars in response to induced drought stress. Environ Exp Bot 81:227–237
Fu J, Huang B (2001) Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45:105–114
Guo Y, Mi F, Yan L, Ren Y, Lv S, Fu B (2014) Physiological response to drought stresses and drought resistances evaluation of different kentucky bluegrass varieties. J Grass Ind 23:220–228
He X, Zeng J, Cao F, Ahmed IM, Zhang G, Vincze E, Wu F (2015) Hvexpb7, a novel beta-expansin gene revealed by the root hair transcriptome of tibetan wild barley, improves root hair growth under drought stress. J Exp Bot 66:7405–7419
Horowitz M, Assadi H (2010) Heat acclimation-mediated cross-tolerance in cardioprotection do hsp70 and hif-1 alpha play a role? Anal Card Dev 1188:199–206
Koyro H-W, Eisa SS (2008) Effect of salinity on composition, viability and germination of seeds of chenopodium quinoa willd. Plant Soil 302:79–90
Lhakdor, Qie B, Liu J (2017) Effects of low temperature, drought, and compound stresses on antioxidant enzyme activities in seedlings of elymus breviaristatus. J Tibet Univ 32:1–6+16.
Li H, Zhao Y (2019) Effect of drought in qiongjie county on crop growth and development. Jiangxi Agric 55
Li X, Huang L, Zhang Y, Hu X (2004) Effects of drought stress on physiological and biochemical indices in five tree species of magnoliaceae. J Plant Resour Environ 13:20–23
Li J, Pu L, Zhu M, Zhang R (2012) The present situation and hot issues in the salt-affected soil research. J Geogr Sci 67:1233–1245
Liu Y, Yan J, Wang P, Wang H (2019) Impacts of climate change on the reclamation of farmers and herdsmen in the tibetan plateau. J Ecol 39:3655–3669
Lu H, Xie H, Lv T, Yao G (2019) Determinants of cultivated land recuperation in ecologically damaged areas in china. Land Use Policy 81:160–166
Noman M, Jameel A, Qiang WD, Ahmad N, Liu WC, Wang FW, Li HY (2019) Overexpression of gmcamta12 enhanced drought tolerance in arabidopsis and soybean. Int J Mol Sci 20:24
Wang X (2010) Principles and techniques of plant physiological and biochemical experiments. Beijing Higher Education Press, Beijing, pp 190–281
Wang J, Kang L, Liu Z, Lv J, Liu Y, Zou X (2017) Research progress on the impact of drought on plant. Hunan Agric Sci:123–126+130.
Wang Y, Xu Q, Yuan H, Zeng X, Nima T (2018) Effects of peg simulated drought stress treatment on growth and physiological characteristics of highland barley seedlings. Barley Cer Sci 35:6–12+42
Yang A, Akhtar SS, Amjad M, Iqbal S, Jacobsen SE (2016) Growth and physiological responses of quinoa to drought and temperature stress. J Agr Crop Sci 202:445–453
Yin CY, Duan BL, Wang X, Li CY (2004) Morphological and physiological responses of two contrasting poplar species to drought stress and exogenous abscisic acid application. Plant Sci 167:1091–1097
Yu L, Wang Y, Garnett T, Auricht G, Han D (2006) A study on physiological responses of varieties of medicago sativa and their relationship with the drought resistance capacity under drought stress. J Grass Ind 15:75–85
Zeng X, Wang Y, Xu Q, Yuan H, Yu M, Dunzhujiabu N (2018) Study on quality properties of hull-less barley. Tibet Agric Sci Technol 40:7–10
Zhai F, Han L, Liu J-x, Qian Y, Ju G, Li W, Zhang S, Sun Z (2013) Assessing cold resistance of mutagenic strains of perennial ryegrass under artificial low-temperature stress. J Grass Ind 22:268–279
Zhang H, Zhao G, Li M, Xia Z, Wang C (2014) Physiological responses of pennisetum longissimumvar.Intermediumseedlings to peg, low temperature and salt stress treatments. J Grass Ind 23:180–188
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This work was sponsored by the National Natural Science Foundation of China (Grant Nos. 31772669 and 31270367).
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Chang, Y., Zhang, J., Bao, G. et al. Physiological Responses of Highland Barley Seedlings to NaCl, Drought, and Freeze-Thaw Stress. J Plant Growth Regul 40, 154–161 (2021). https://doi.org/10.1007/s00344-020-10085-5
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DOI: https://doi.org/10.1007/s00344-020-10085-5