Abstract
Caspase-8, first classified as a pro-apoptotic caspase, is considered to have arisen from duplication with caspase-10 and involves multiple immune and inflammatory responses in mammals. However, few are known on the phylogeny and function of caspase-8 in molluscs, one of the largest phyla in marine invertebrates. In this study, we conducted phylogenetic and functional analysis on molluscan caspase-8-like genes. Results indicate that duplication occurred in molluscan caspase-8-like genes, resulting in at least two caspase-8 copies in some groups of bivalves. Additional studies in Pacific oyster Crassostrea gigas showed different spatio-temporal expression patterns and subcellular localizations of CgCaspase-8-1 and CgCaspase-8-2. While no interaction was observed between CgCaspase-8-2 and CgFADD, the adaptor molecule in apoptosis, yeast two-hybrid and co-immunoprecipitation assays suggested the interaction between CgCaspase-8-1 and CgFADD, indicating its pro-apoptotic function. In addition, CgCaspase-8-1 showed interaction with the CARD domain of CgRIG-I. Together with two NF-κB subunits (Cgp105 and CgRel), their transcripts were up-regulated in response to poly(I:C) stimuli, supporting the immune function of both pro, and anti-inflammation. The results provide insight into the evolution and functional diversification of Bivalvia caspase-8 genes.
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Data Availability Statement
The data used and analyzed during this study are included in this article or available from the corresponding author on reasonable request.
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We thank Jie MENG for her valuable suggestions and comments on this manuscript, and Oceanographic Data Center, IOCAS, for data support.
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Supported by the Science & Technology Innovation Project of Laoshan Laboratory (No. LSKJ202203001), the Center for Ocean Mega-Research of Science, Chinese Academy of Sciences (No. COMS2019Q11), the GHfund B (No. 20210702), and the Taishan Scholars Program
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Deng, S., Qu, T., Zhang, G. et al. Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes. J. Ocean. Limnol. 41, 1109–1121 (2023). https://doi.org/10.1007/s00343-022-2129-7
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DOI: https://doi.org/10.1007/s00343-022-2129-7