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CgPG21 is involved in the degradation of the cell wall during the secretory cavity formation in Citrus grandis ‘Tomentosa’ fruits

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Abstract

Main conclusion

CgPG21 is mainly located in the cell wall, participates in the intercellular layer degradation of the cell wall during the formation of secretory cavity in the intercellular space-forming and lumen-expanding stages.

Abstract

The secretory cavity is a common structure in Citrus plants and is the main site for synthesis and accumulation of medicinal ingredients. The secretory cavity is formed in lysogenesis, when epithelial cells enter a process of programmed cell death. Pectinases are known to be involved in degradation of the cell wall during the cytolysis of secretory cavity cells, but the changes in cell structure, the dynamic characteristics of cell wall polysaccharides and the related genes regulating cell wall degradation are unclear. In this study, electron microscopy and cell wall polysaccharide-labeling techniques were used to study the main characteristics of cell wall degradation of the secreting cavity of Citrus grandis ‘Tomentosa’ fruits. At the same time, the full CDS length of the pectinase gene CgPG21 was cloned, encoding a protein composed of 480 amino acids. CgPG21 is mainly located in the cell wall, participates in the degradation of the intercellular layer of the cell wall during the development of the secretory cavity, and plays an important role in the formation of the secretory cavity in the intercellular space-forming and lumen-expanding stages. With the development of secretory cavity, the cell wall polysaccharides of epithelial cells gradually degrade. CgPG21 is mainly involved in the intercellular layer degradation.

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All data generated or analyzed during this study are included in this published article [and its supplementary information files].

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (project no. 32270381 to MB, 31870172 to HW), Natural Science Foundation of Guangdong (project no. 2022A1515011086 to MB), the Open Competition Program of Ten Major Directions of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province (project no. 2022SDZG07 to HW), Laboratory of Lingnan Modern Agriculture Project (project no. NZ 2021024 to HW), Key Realm R&D Program of Guangdong Province (project no. 2020B020221001 to HW), Agricultural Science and Technology Innovation and Promotion Project of Guangdong (project no. 2018LM2160 to HW), and Research Fund of Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture (No. 2022KF009).

Funding

This work was supported by the National Natural Science Foundation of China (project no. 32270381 to MB), Natural Science Foundation of Guangdong (project no. 2022A1515011086 to MB), National Natural Science Foundation of China (project no. 31870172 to HW), the Open Competition Program of Ten Major Directions of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province, (project no. 2022SDZG07 to HW), Laboratory of Lingnan Modern Agriculture Project (project no. NZ 2021024 to HW), Key Realm R&D Program of Guangdong Province (project no. 2020B020221001 to HW), Agricultural Science and Technology Innovation and Promotion Project of Guangdong (project no. 2018LM2160 to HW), Research Fund of Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture (No. 2022KF009 to MB).

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Author notes

  1. Mei Bai, Panpan Tong and Qun Luo are Contributed equally to this work.

Authors and Affiliations

  1. Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Natural Medicine, South China Agricultural University, Guangzhou, 510642, China

    Mei Bai, Panpan Tong, Qun Luo, Na Shang, Hailan Huang, Bin Huai & Hong Wu

  2. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China

    Mei Bai & Hong Wu

  3. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, China

    Mei Bai & Hong Wu

  4. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Hong Wu

  5. Maoming Branch Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, 525000, China

    Mei Bai & Hong Wu

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  1. Mei Bai
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Contributions

HW and MB conceived the project. HW and MB designed the experiments. PT, NS, HH and BH performed the experiments and the data analysis. MB and HW wrote the manuscript, QL revised the manuscript.

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Correspondence to Mei Bai or Hong Wu.

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Bai, M., Tong, P., Luo, Q. et al. CgPG21 is involved in the degradation of the cell wall during the secretory cavity formation in Citrus grandis ‘Tomentosa’ fruits. Plant Cell Rep 42, 1311–1331 (2023). https://doi.org/10.1007/s00299-023-03032-7

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