Abstract
Key message
RbIDL1 and RbIDL4 are up-regulated in an ethylene-responsive manner during rose petal abscission and restored the Arabidopsis ida-2 mutant abscission defect suggesting functional conservation of the IDA pathway in rose.
Abstract
Abscission is an ethylene-regulated developmental process wherein plants shed unwanted organs in a controlled manner. The INFLORESCENCE DEFICIENT IN ABSCISSION family has been identified as a key regulator of abscission in Arabidopsis, encoding peptides that interact with receptor-like kinases to activate abscission. Loss of function ida mutants show abscission deficiency in Arabidopsis. Functional conservation of the IDA pathway in other plant abscission processes is a matter of interest given the discovery of these genes in several plants. We have identified four members of the INFLORESCENCE DEFICIENT IN ABSCISSION-LIKE family from the ethylene-sensitive, early-abscising fragrant rose, Rosa bourboniana. All four are conserved in sequence and possess well-defined PIP, mIDa and EPIP motifs. Three of these, RbIDL1, RbIDL2 and RbIDL4 show a three–fourfold increase in transcript levels in petal abscission zones (AZ) during ethylene-induced petal abscission as well as natural abscission. The genes are also expressed in other floral tissues but respond differently to ethylene in these tissues. RbIDL1 and RbIDL4, the more prominently expressed IDL genes in rose, can complement the abscission defect of the Arabidopsis ida-2 mutant; while, promoters of both genes can drive AZ-specific expression in an ethylene-responsive manner even in Arabidopsis silique AZs indicating recognition of AZ-specific and ethylene-responsive cis elements in their promoters by the abscission machinery of rose as well as Arabidopsis.
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Acknowledgements
We are grateful to the University Grants Commission for research fellowships to PS and SKM and the Council of Scientific and Industrial Research, India for financial support to APS (under BSC107) for the work. We are grateful to Mr Ram Awadh for taking care of the rose plants.
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This work was funded by CSIR-National Botanical Research Institute, BSC107, UGC-DAE Consortium for Scientific Research, University Grants Commission.
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APS conceived the experiment. PS, SKM, DS carried out the experiments. PS and APS analyzed the data and wrote the paper. All authors read and approved the manuscript.
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Communicated by Prakash P. Kumar.
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Supplementary Fig.1. a Quantitative real time PCR analysis of various RbIDL members during ethylene-induced and natural petal abscission and b in petal AZs of flowers treated with 15 µl L-1 ethylene. Expression values were analyzed as mentioned above
Supplementary Fig. 2. Quantitative real time PCR analysis of RbIDLs expression in different tissues. Expression values were analyzed as mentioned above
Supplementary Fig. 3. Expression of RbIDL1pro and RbIDL4pro in transgenic Arabidopsis plants.Histochemical GUS expression analysis of RbIDL1pro and RbIDL4pro in 3 days old Arabidopsis seedling (a and b) and of RbIDL1pro in one month old plant leaf (a) under ethylene-untreated and 0.5 µl L-1 ethylene-treated conditions
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Singh, P., Maurya, S.K., Singh, D. et al. The rose INFLORESCENCE DEFICIENT IN ABSCISSION-LIKE genes, RbIDL1 and RbIDL4, regulate abscission in an ethylene-responsive manner. Plant Cell Rep 42, 1147–1161 (2023). https://doi.org/10.1007/s00299-023-03017-6
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DOI: https://doi.org/10.1007/s00299-023-03017-6