Abstract
Key message
Chemical agents such as trichostatin A (TSA) can assist in optimization of doubled haploidy for rapid improvements in wheat germplasm and addressing recalcitrance issues in cell culture responses.
Abstract
In wheat, plant regeneration through microspore culture is an integral part of doubled haploid (DH) production. However, low response to tissue culture and genotype specificity are two major constraints in the broad deployment of this breeding tool. Recently, the structure of chromatin was shown to be linked with cell transitions during tissue culture. Specifically, repression of genes that are required for cell morphogenesis, through acetylation of histones, may play an important role in this process. Reduction of histone acetylation by chemical inhibition may increase tissue culture efficiency. Here, the role of trichostatin A (TSA) in inducing microspore-derived embryos was investigated in wheat. The optimal dose of TSA was determined for wheat cultivars and subsequently validated in F1 hybrids. A significant increase in the efficiency of DH production was observed in both cultivated varieties and F1 hybrids. Thus, the inclusion of TSA in DH protocols for wheat breeding programs is advocated.
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Asif M, Eudes F, Randhawa H, Amundsen E, Yanke J, Spaner D (2013) Cefotaxime prevents microbial contamination and improves microspore embryogenesis in wheat and triticale. Plant Cell Rep 32(10):1637–1646
Asif M, Eudes F, Randhawa H, Amundsen E, Spaner D (2014) Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat. Plant Cell Tissue Organ Cult 116(1):125–130
Chang S, Pikaard CS (2005) Transcript profiling in Arabidopsis reveals complex responses to global inhibition of DNA methylation and histone deacetylation. J Biol Chem 280(1):796–804
Delporte F, Jacquemin JM, Masson P, Watillon B (2012) Insights into the regenerative property of plant cells and their receptivity to transgenesis: wheat as a research case study. Plant Signal Behav 7(12):1608–1620
Forster BP, Thomas WTB (2010) Doubled haploids in genetics and plant breeding. Plant breeding reviews. Wiley, New York, pp 57–88
Haberer G, Mayer KF, Spannagl M (2016) The big five of the monocot genomes. Curr Opin Plant Biol 30:33–40
Hussain B, Khan MA, Ali Q, Shaukat S (2012) Why double haploid production is the best method for genetic improvement and genetic studies of wheat? IJAVMS 6(4):216–228
Islam SM, Tuteja N (2012) Enhancement of androgenesis by abiotic stress and other pretreatments in major crop species. Plant Sci 182:134–144
Li H, Soriano M, Cordewener J, Muino JM, Riksen T, Fukuoka H, Angenent GC, Boutilier K (2014) The histone deacetylase inhibitor trichostatin a promotes totipotency in the male gametophyte. Plant Cell 26(1):195–209
Marks PA, Richon VM, Rifkind RA (2000) Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells. J Natl Cancer Inst 92(15):1210–1216
Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127(4):519–534
Sinha RK, Eudes F (2015) Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores. Plant Cell Tissue Organ Cult 122(1):227–237
Yang F, Zhang L, Li J, Huang J, Wen R, Ma L, Zhou D, Li L (2010) Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize. BMC Plant Biol 10(1):178
Yoshida M, Kijima M, Akita M, Beppu T (1990) Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. J Biol Chem 265(28):17174–17179
Acknowledgements
We acknowledge Syngenta and KWS for providing wheat cultivars used in this study. We thank Western Grains Research Foundation for funding this research project. Activities related to F1 hybrids were co-funded by Alberta Innovates Biosolutions, Western Grains Research Foundation, Alberta Wheat Commission and the Alberta Crop Improvement Development Fund.
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Communicated by Attila Feher.
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Jiang, F., Ryabova, D., Diedhiou, J. et al. Trichostatin A increases embryo and green plant regeneration in wheat. Plant Cell Rep 36, 1701–1706 (2017). https://doi.org/10.1007/s00299-017-2183-3
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DOI: https://doi.org/10.1007/s00299-017-2183-3