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Isolation and functional characterization of a novel rice constitutive promoter

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Abstract

Key message

A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering.

Abstract

Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P OsCon1 ), from the 5′ upstream region of a constitutively expressed rice gene OsDHAR1. In P OsCon1 ::GUS transgenic rice, we showed that P OsCon1 had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P OsCon1 exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P OsCon1 activity was not affected by developmental stages or by environmental factors. Further, 5′-deletions analysis indicated that the distinct regions might contribute to the strong expression of P OsCon1 in different tissues. Overall, our results suggest that P OsCon1 is a novel constitutive promoter, which could potentially use in transgenic crop development.

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Abbreviations

P OsCon1 :

Rice constitutive promoter 1

P OsAct1 :

Rice Actin1 promoter

P 35S :

Cauliflower mosaic virus 35S promoter

P ZmUbi :

Maize ubiquitin promoter

P OsCc1 :

Rice OsCc1 promoter

DHAR:

Dehydroascorbate reductase

GUS:

β-Glucuronidase

qRT-PCR:

Quantitative reverse transcription polymerase chain reaction

MS:

Murashige and Skoog

X-Gluc:

5-Bromo-4-chloro-3-indolyl-beta-glucuronide

4-MU:

4-Methylumbelliferone

DAG:

Days after germination

ASF1:

Activating sequence factor 1

ASF2:

Activating sequence factor 2

EEC:

Enhancer element consensus

CGF1:

Chlorophyll a/b-binding protein gene GATA-box factor 1

DHA:

Dehydroascorbate

cytDHAR:

Cytosolic DHAR

AsA:

Ascorbate acid

ROS:

Reactive oxygen species

SD:

Standard deviation

GAP:

Glyceraldydyde-3-phosphate dehydrogenase

CAB:

Chlorophyll A/B binding protein

RBCS:

Ribulose bisphosphate carboxylase

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Acknowledgments

We thank Dr. Changlin Wang (Washington State University) for help with the manuscript. This research was supported by the National Natural Science Foundation of China (NSFC Grant No. 31100216) and by the Creative Foundation of the Anhui Agricultural Academy of Sciences (13C0101 & 14B0113).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei, 230031, China

    Juan Li & Jian-Bo Yang

  2. Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    Juan Li, Rong-Fang Xu, Rui-Ying Qin, Hui Ma, Hao Li, Ying-Ping Zhang, Li Li, Peng-Cheng Wei & Jian-Bo Yang

  3. Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    Juan Li, Hao Li, Peng-Cheng Wei & Jian-Bo Yang

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  1. Juan Li
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Correspondence to Peng-Cheng Wei.

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Communicated by Huw D. Jones.

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Li, J., Xu, RF., Qin, RY. et al. Isolation and functional characterization of a novel rice constitutive promoter. Plant Cell Rep 33, 1651–1660 (2014). https://doi.org/10.1007/s00299-014-1644-1

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