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Histological and cytological characterization of adult plant resistance to wheat stripe rust

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Abstract

Wheat cultivar Xingzi 9104 (XZ) possesses adult plant resistance (APR) to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). In this study, histological and cytological experiments were conducted to elucidate the mechanisms of APR in XZ. The results of leaf inoculation experiments indicated that APR was initiated at the tillering stage, gradually increased as the plant aged and highly expressed after boot stage. The histology and oxidative burst in infected leaves of plants at seedling, tillering and boot stages were examined using light microscopic and histochemical methods. Subcellular changes in the host–pathogen interactions during the seedling and boot stages were analyzed by transmission electron microscopy. The results showed that haustorium formation was retarded in the adult plants and that the differentiation of secondary intercellular hyphae was significantly inhibited, which decreased the development of microcolonies in the adult plants, especially in plants of boot stage. The expression of APR to stipe rust during wheat development was clearly associated with extensive hypersensitive cell death of host cells and localized production of reactive oxygen species, which coincided with the restriction of fungal growth in infection sites in adult plants. At the same time, cell wall-related resistance in adult plants prevented ingression of haustorial mother cells into plant cells. Haustorium encasement was coincident with malformation or death of haustoria. The results provide useful information for further determination of mechanisms of wheat APR to stripe rust.

Key message The expression of APR to stipe rust in wheat cultivar Xingzi 9104 (XZ) was clearly associated with extensive hypersensitive cell death of host cells and the localized production of reactive oxygen species.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No. 30930064), the Earmark Fundof China Agriculture Research System (No. CARS-3-1-11), and the 111 Project from the Ministry of Education of China (No. B07049).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Hongchang Zhang, Chenfang Wang, Yulin Cheng, Qingmei Han, Lili Huang, Guorong Wei & Zhensheng Kang

  2. College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Hongchang Zhang

  3. USDA-ARS, Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    Xianming Chen

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  1. Hongchang Zhang
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Correspondence to Zhensheng Kang.

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Communicated by Q. Zhao.

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Zhang, H., Wang, C., Cheng, Y. et al. Histological and cytological characterization of adult plant resistance to wheat stripe rust. Plant Cell Rep 31, 2121–2137 (2012). https://doi.org/10.1007/s00299-012-1322-0

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  • DOI: https://doi.org/10.1007/s00299-012-1322-0

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