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Regeneration of Acer caudatifolium Hayata plantlets from juvenile explants

  • Cell Biology and Morphogenesis
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Abstract

Juvenile and fully mature Acer caudatifolium Hayata explants were assayed for their organogenic capacity. A protocol for multiple shoot culture formation and in vitro plant regeneration was developed for juvenile axillary bud cultures. Mature explants failed in shoot regeneration. Shoot multiplication was achieved by releasing apical dominance of the single elongated shoot on woody plant medium (WPM) supplemented with 0.7 mg l−1 6-benzylaminopurine and 0.05 mg l−1 α-naphthaleneacetic acid. The highest rooting percentage was recorded on half-strength WPM containing 1.0 mg l−1 indole-3-butyric acid. Regenerated plantlets were successfully hardened to ex vitro conditions and continued to grow after transfer to soil. No morphological aberrations were observed in the regenerates.

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Fig. 1a–f.

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Abbreviations

BAP :

6-Benzylaminopurine

IBA :

Indole-3-butyric acid

NAA :

α-Naphthaleneacetic acid

PPM :

Plant preservative mixture

TDZ :

Thidiazuron

WPM :

Woody plant medium

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Acknowledgements

The author thanks Dr Andrej Kormuťák for providing maple seeds, Dr Jeng-Der Chung for sharing the essential literature, Dr Branko Slobodník for graphic assistance, and Dr Dušan Gömöry and Professor Ladislav Paule for helpful comments. The excellent laboratory assistance of Helena Parobková and Zuzana Slančíková is acknowledged. This work was financed by the Slovak Grant Agency VEGA 1/4036/97 and 1/7056/20.

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  1. Department of Phytology, Technical University, Masarykova 24, 960 53, Zvolen, Slovakia

    J. Ďurkovič

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  1. J. Ďurkovič
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Correspondence to J. Ďurkovič.

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Communicated by D. Bartels

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Ďurkovič, J. Regeneration of Acer caudatifolium Hayata plantlets from juvenile explants. Plant Cell Rep 21, 1060–1064 (2003). https://doi.org/10.1007/s00299-003-0634-5

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  • DOI: https://doi.org/10.1007/s00299-003-0634-5

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