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Mrc1/Claspin: a new role for regulation of origin firing

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Abstract

Mrc1 and its vertebrate homologue Claspin serve as a mediator for replication stress checkpoint signaling, receiving the signal from Mec1/Rad3/ATR sensor kinase and transmitting it to the effector Rad53/Cds1/Chk1 kinase. They are likely to be a part of the replisome and facilitate the S-phase progression by promoting replication fork progression. Recent reports on Mrc1/Claspin indicate their new role in regulating the replication initiation through interaction with Cdc7, a key conserved serine–threonine kinase that triggers firing at each replication origin. Mrc1/Claspin has a specific domain that specifically interacts with Cdc7, and this domain is involved also in intramolecular interaction with its N-terminal segment. Mechanisms for novel regulation of origin firing and its timing through recruitment of Cdc7 to Mrc1/Claspin will be discussed.

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Acknowledgements

We would like to thank all the members of our laboratory and other collaborators for helpful discussion, support, and continuous excitement.

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Authors and Affiliations

  1. Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan

    Hisao Masai, Chi-Chun Yang & Seiji Matsumoto

Authors
  1. Hisao Masai
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  2. Chi-Chun Yang
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  3. Seiji Matsumoto
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Corresponding author

Correspondence to Hisao Masai.

Additional information

Communicated by M. Kupiec.

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Masai, H., Yang, CC. & Matsumoto, S. Mrc1/Claspin: a new role for regulation of origin firing. Curr Genet 63, 813–818 (2017). https://doi.org/10.1007/s00294-017-0690-y

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  • DOI: https://doi.org/10.1007/s00294-017-0690-y

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