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Comprehensive Genomic Analysis Reveals Extensive Diversity of Type I and Type IV Secretion Systems in Klebsiella pneumoniae

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Abstract

The diversity and distribution of secretion systems in Klebsiella pneumoniae are unclear. In this study, the six common secretion systems (T1SS–T6SS) were comprehensively investigated in the genomes of 952 K. pneumoniae strains. T1SS, T2SS, type T subtype of T4SS, T5SS, and subtype T6SSi of T6SS were found. The findings indicated fewer types of secretion systems in K. pneumoniae than reported in Enterobacteriaceae, such as Escherichia coli. One conserved T2SS, one conserved T5SS, and two conserved T6SS were detected in more than 90% of the strains. In contrast, the strains displayed extensive diversity of T1SS and T4SS. Notably, T1SS and T4SS were enriched in the hypervirulent and classical multidrug resistance pathotypes of K. pneumoniae, respectively. The results expand the epidemiological knowledge of the virulence and transmissibility of pathogenic K. pneumoniae and contribute to identify the potential strains for safe applications.

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Acknowledgements

We want to acknowledge all the laboratory members at the bioengineering department, the East China University of Science and Technology who help provide the requirements of scientific research.

Funding

This work was supported by the National Natural Science Foundation of China under Grant No. 31271862.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Menglei Yang, Xiaoqin Zhou, Yangyang Bao, Yang Zhang, Boya Liu, Luxi Gan, Weihua Tao, Jinyou Tuo & Heng Gong

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Contributions

MY, XZ and HG conceived and designed the study. MY, YB and YZ collected the data and performed the sequence analysis. BL, LG, WT and JT check the data of this study. MY and HG wrote the main manuscript text. All authors reviewed the manuscript.

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Correspondence to Heng Gong.

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Table S1

Information of 952 Klebsiella pneumoniae strains. Supplementary file1 (XLSX 233 KB)

Table S2

Secretion systems identified in 952 Klebsiella pneumoniae strains, and the co-existing genes in the corresponding secretion system cluster used for phylogenetic analysis. Supplementary file2 (XLSX 233 KB)

Table S3

Number of virulence genes and resistant genes in 952 Klebsiella pneumoniae strains. Supplementary file3 (XLSX 61 KB)

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Yang, M., Zhou, X., Bao, Y. et al. Comprehensive Genomic Analysis Reveals Extensive Diversity of Type I and Type IV Secretion Systems in Klebsiella pneumoniae. Curr Microbiol 80, 270 (2023). https://doi.org/10.1007/s00284-023-03362-5

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