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Effect of Limosilactobacillus reuteri ZJF036 on Growth Performance and Gut Microbiota in Juvenile Beagle Dogs

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Abstract

This experiment investigated the effects of Limosilactobacillus reuteri ZJF036 on growth performance, serum biochemical parameters, and gut microbiota in beagle dogs. Sixteen 75 ± 5-day-old healthy male beagles (4.51 ± 1.37 kg) were randomly divided into two groups; the experimental group (L1) and the control group (L0), and then fed with or without a basal diet containing L. reuteri ZJF036 (109 CFU/g), respectively. The results showed that there was no significant difference in daily weight gain between the two groups (P > 0.05). However, we found that L. reuteri ZJF036 decreased Chao1 index and ACE index and increased the relative abundance of Firmicutes and Fusobacteria (P < 0.05) compared to the L0 group. In addition, we also found that the ratio of Firmicutes to Bacteroidetes was decreased in L1 group. Furthermore, the relative abundance of Lactobacillus increased, while that of Turicibacter and Blautia decreased in L1 group (P < 0.05). In conclusion, L. reuteri ZJF036 appeared to regulate the intestinal microbiota of beagle dogs. This study revealed the potential use of L. reuteri ZJBF036 as a probiotic supplement for beagle dogs.

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Acknowledgements

All authors are thankful to Institute of special animal and plant sciences of Chinese Academy of Agricultural Sciences.

Funding

This study was supported by the Natural Science Foundation of Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2021-ISAPS) and the Inner Mongolia Autonomous Region Science and Technology Plan Project (2022YFDZ0072).

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Authors and Affiliations

  1. College of Agriculture, Chifeng University, Chifeng, 024000, People’s Republic of China

    Dehui Zhao & Hanlu Liu

  2. Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, People’s Republic of China

    Dehui Zhao & Haihua Zhang

  3. Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun, 130112, People’s Republic of China

    Dehui Zhao, Ruchun Zhang, Jinming Wang & Xinyu Zhang

  4. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, People’s Republic of China

    Keyuan Liu

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  1. Dehui Zhao
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  4. Xinyu Zhang
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Contributions

DZ conceived the experiment, analyzed the data, and wrote the manuscript. DZ, RZ, JW, and XZ collected the samples and conducted laboratory analyses. KL conducted the animal trial. HL and HZ designed the whole trial and reviewed the manuscript. All authors approved the final manuscript as submitted.

Corresponding author

Correspondence to Hanlu Liu.

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The Authors declare that there is no conflict of interest regarding the publication of this paper.

Ethical Approval

All animal use was approved by The Animal Administration and Ethics Committee of Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences (Permit No. ISAPSAEC-2021-001B).

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Zhao, D., Zhang, R., Wang, J. et al. Effect of Limosilactobacillus reuteri ZJF036 on Growth Performance and Gut Microbiota in Juvenile Beagle Dogs. Curr Microbiol 80, 155 (2023). https://doi.org/10.1007/s00284-023-03276-2

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