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Lysobacter sedimenti sp. nov., Isolated from the Sediment, and Reclassification of Luteimonas lumbrici as Lysobacter lumbrici comb. nov

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Abstract

A bacterium, designated 50T was isolated from the sediment of a pesticide plant in Shandong Province, PR China. The strain was non-motile, Gram stain-negative, rod shaped and grew optimally on NA medium at 30 °C, pH 7.5 and with 0% (w/v) NaCl. Strain 50T showed the highest 16S rRNA gene sequence similarity with Lysobacter pocheonensis Gsoil 193T (96.7%), followed by Luteimonas lumbrici 1.1416T (96.5%). Phylogenetic analyses based on 16S rRNA indicated that strain 50T and Luteimonas lumbrici 1.1416T were clustered with the genus of Lysobacter and formed a subclade with Lysobacter pocheonensis Gsoil 193T. In the phylogenetic analysis based on the genome sequences, strain 50T and Luteimonas lumbrici 1.1416T were also clustered with the type strains of the genus Lysobacter. The obtained ANI and the dDDH value between 50T and Luteimonas lumbrici 1.1416T were 80.6% and 24.0%, respectively. The respiratory quinone was ubiquinone-8 (Q-8), and the major cellular fatty acids were iso-C15: 0 (31.7%), summed feature 9 (iso-C17:1 ω9c or C16:0 10-methyl) (23.7%), iso-C17:0 (14.3%) and iso-C16:0 (12.6%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified aminophospholipid, unidentified phospholipid and unidentified lipid. The genomic DNA G + C content was 69.5 mol%. According to the phenotypic, chemotaxonomic and phylogenetic analyses, strain 50T represents a novel species of the genus Lysobacter, for which the name Lysobacter sedimenti sp. nov. is proposed, with strain 50T (= KCTC 92088T = CCTCC AB 2022035T) as the type strain. In this study, it is also proposed that Luteimonas lumbrici should be transferred to the genus Lysobacter as Lysobacter lumbrici comb. nov. The type strain of Lysobacter lumbrici is 1.1416T (= KCTC 62979T = CCTCC AB 2018348T).

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31800097 and 32170128).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Xuan Zhang, Ningning Wu, Keke Geng & Jian He

  2. College of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou, 466000, Henan, People’s Republic of China

    Pan Yang & Cuiwei Chu

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  1. Xuan Zhang
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  2. Ningning Wu
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Contributions

Conceptualization, funding acquisition and supervision: CC and JH; Laboratory work, data analysis and writing of the original draft: XZ; Writing, reviewing and editing of the manuscript: NW, KG and YP. All authors read and approved the final manuscript.

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Correspondence to Cuiwei Chu or Jian He.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and the whole genome of strain 50T are ON037515 and JALGCL000000000, respectively.

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Zhang, X., Wu, N., Geng, K. et al. Lysobacter sedimenti sp. nov., Isolated from the Sediment, and Reclassification of Luteimonas lumbrici as Lysobacter lumbrici comb. nov. Curr Microbiol 79, 381 (2022). https://doi.org/10.1007/s00284-022-03084-0

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