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In Vitro Study of the Effect of Inhibition of Quorum Sensing by Brominated Furanone on Peritoneal Dialysis-Associated Peritonitis Associated with Escherichia Coli Infection

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Abstract

In recent years, the occurrence of peritoneal dialysis (PD)-associated peritonitis (PDAP) with Escherichia coli infection has gradually increased. The presence of quorum sensing (QS) among bacteria facilitates the expansion of antibiotic resistance. Brominated furanone (BMF), a halogenated furanone compound isolated from macroalgae, is a new type of quorum-sensing inhibitor that can inhibit bacterial quorum sensing and reduce bacterial resistance. In this study, we established an in vitro peritoneal dialysis-associated peritonitis biofilm model. After intervention with BMF, the biofilm was destroyed, as shown by scanning electron microscopy, and the number of viable bacteria was reduced. Crystal violet semiquantitative determination showed that biofilm absorption significantly decreased, and RT–PCR showed that luxS expression was downregulated after drug intervention. Therefore, we propose that BMF can effectively inhibit E. coli QS by disrupting the bacterial biofilm and downregulating QS gene expression to reduce the bacterial resistance, providing a direction for the development of novel antibacterial drugs.

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Acknowledgements

The authors thank Han Jiahui at The First Affiliated Hospital of Guangxi Medical University for his help in analyzing the data. Thanks to teacher Zhen from Guangxi Medical University for helping us with the electron microscope.

Funding

This study was supported by the Natural Science Fund of Guangxi, P.R (2018GXNSFAA050084).

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Authors and Affiliations

  1. Department of Nephrology, the First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, China

    Jinqiu Li, Xiaofang Wei, Yashan Song, Xiaohua Li & Chengyu Wang

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  1. Jinqiu Li
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  2. Xiaofang Wei
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  3. Yashan Song
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  4. Xiaohua Li
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  5. Chengyu Wang
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Contributions

Dr. Wang designed and organized the study and revised the manuscript. Dr. Li and Dr. Wei established the in vitro static biofilm model, detected carrier biofilms by SEM, identified biofilms by crystal violet staining, detected luxS gene expression by RT–PCR, analyzed the data, and drafted the manuscript. Dr. Song and Dr. Li critically revised the manuscript. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Chengyu Wang.

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Conflict of interest

All authors declared that they have no confict of interest.

Ethical Approval

This study was approved by the Ethics Committee of the First Affiliated Hospital of Guangxi Medical University[Approval Number: NO.2022-KY-E-(059)], Nanning, China.

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Li, J., Wei, X., Song, Y. et al. In Vitro Study of the Effect of Inhibition of Quorum Sensing by Brominated Furanone on Peritoneal Dialysis-Associated Peritonitis Associated with Escherichia Coli Infection. Curr Microbiol 79, 337 (2022). https://doi.org/10.1007/s00284-022-03040-y

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