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Salinimicrobium sediminilitoris sp. nov., Isolated from a Tidal Flat

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Abstract

A Gram-negative, facultatively anaerobic, motile, and rod-shaped bacterium, designated ASW11-47 T, was isolated from a tidal flat sediment taken from the coast of Qingdao, PR China. Phylogenetic analysis of 16S rRNA gene sequence showed that strain ASW11-47 T belongs to the genus Salinimicrobium and is most closely related to Salinimicrobium terrae YIM-C338T (98.68% similarity). The length of draft genome is 3,594,457 bp, and DNA G + C content is 40.8 mol%. The values of average nucleotide identity and digital DNA–DNA hybridization between strain ASW11-47 T and closely related strains were in ranges of 75.9–85.9 and 19.7–31.5%, respectively. The major fatty acids (> 10%) were iso-C15:0 and iso-C17:0 3-OH. The predominant respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. On the basis of genotypic, phenotypic, and chemotaxonomic analysis, strain ASW11-47 T represents a novel species within the genus Salinimicrobium, for which the name Salinimicrobium sediminilitoris sp. nov. is proposed. The type strain is ASW11-47 T (= KCTC 82501 T = MCCC 1K05586T).

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Data Availability

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and the genome sequence of strain ASW11-47 T are OK161349 and JAIVCC000000000, respectively.

Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

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Funding

This work was supported by the Youth Foundation of the Shanxi Science and Technology Department (201901D211375), National Natural Science Foundation of China (32002143, 31972590), Excellent Talents Come to Shanxi to Reward Scientific Research Projects (SXYBKY2019024, SXYBKY2019025), Science and Technology Innovation Fund Project of Shanxi Agricultural University (2020BQ07), Animal Husbandry and ‘1331 project’ Key Discipline Construction program of Shanxi Province.

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  1. Cheng-Qiang Xia and Hui-Jing Niu have contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science, Shanxi Agricultural University, Taigu, 030801, China

    Cheng-Qiang Xia, Hui-Jing Niu, Li-Ping Sun, Qin Wang & Cai-Xia Pei

  2. College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China

    Kai-Shi Dong, Li Guan & Yi Li

  3. Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China

    Yan-Jiao Zhang

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Contributions

CQX analyzed the data and wrote and revised the manuscript; HJN performed the experiments and wrote the manuscript; KSD, LG, and YJZ carried out chemotaxonomic analysis; LPS and QW performed phylogenetic analysis; YL and CXP designed and supervised the study. All authors read and approved the final version of the manuscript.

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Correspondence to Cheng-Qiang Xia, Yi Li or Cai-Xia Pei.

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Xia, CQ., Niu, HJ., Dong, KS. et al. Salinimicrobium sediminilitoris sp. nov., Isolated from a Tidal Flat. Curr Microbiol 79, 350 (2022). https://doi.org/10.1007/s00284-022-03037-7

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