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RNA-Seq Transcriptomic Analysis of Green Tea Polyphenols Modulation of Differently Expressed Genes in Enterococcus faecalis Under Bile Salt Stress

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Abstract

Enterococcus faecalis (E. faecalis) belongs to lactic acid bacteria which can be used as a probiotic additive and feed, bringing practical value to the health of humans and animals. The prebiotic function of tea polyphenols lays a foundation for green tea polyphenols (GTP) to repair the adverse changes of E. faecalis under stress conditions. In this study, RNA-sequence analysis was used to explore the protective effect of GTP on E. faecalis under bile salt stress. A total of 50 genes were found to respond to GTP under bile salts stress, containing 18 up-regulated and 32 down-regulated genes. The results showed that multiple genes associated with cell wall and membrane, transmembrane transport, nucleotide transport and metabolism were significantly differentially expressed (P < 0.05), while GTP intervention can partly alleviate the detrimental effects of bile salt on amino acid metabolism and transport. The present study provides the whole genome transcriptomics of E. faecalis under bile salt stress and GTP intervention which help us understand the growth and mechanism of continuous adaptation of E. faecalis under stress conditions.

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Data Availability

The authors confirm that all data generated or analyzed during this study are included in this published article [and its supplementary information files]. The NCBI BioProject is PRJNA796179 [Transcriptome sequencing of Enterococcus faecalis].

Abbreviations

E. faecalis :

Enterococcus faecalis

E. faecalis 131-2:

Enterococcus faecalis131-2

GTP:

Green tea polyphenols

GO:

Gene ontology

KEGG:

Kyoto encyclopedia of genes and genomes

SEM:

Scanning electron microscopy

DEGs:

Differentially expressed genes

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Funding

This work was supported by the Fundamental Research Funds for the Central Universities (2020SKTY01), the National Natural Science Foundation of China (31650006), "Yueqi Young Scholars" Funding Program of China University of Mining and Technology (Beijing), and the Key Research and Development Project of Zhejiang Province (2018C02047).

Author information

Authors and Affiliations

  1. Department of Physical Education, China University of Mining and Technology, Beijing, 100083, People’s Republic of China

    Li Zhang, Jingyi Xie & Zhenying Zhang

  2. Department of Food Science and Engineering, Ningbo University, Ningbo, 315211, People’s Republic of China

    Ruonan Yan & Zufang Wu

Authors
  1. Li Zhang
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  2. Jingyi Xie
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  3. Zhenying Zhang
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Contributions

LZ and JX drafted the manuscript. LZ and ZZ analyzed the RNA sequencing data. LZ, ZW, and RY conducted the statistics study. All authors critically revised the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Zufang Wu.

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Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (JPG 332 KB)

Supplementary file2 (JPG 116 KB)

Supplementary file3 (JPG 63 KB)

Supplementary file4 (XLSX 11 KB)—Primer sequence of the representative RNA-seq DEGs

Supplementary file5 (XLSX 10 KB)—Mapping ratio statistics

Supplementary file6 (XLS 252 KB)—DEGs between the bile salt group and the control group

Supplementary file7 (XLS 37 KB)—DEGs between the bile salt-GTP and the bile salt group

Supplementary file8 (XLS 185 KB)—GO analysis of DEGs between the bile salt group and the control group

Supplementary file9 (XLS 5 KB)—GO analysis of DEGs between the bile salt-GTP group and the bile salt group

Supplementary file10 (XLSX 14 KB)—All genes between the bile salt-GTP group and the bile salt group

Supplementary file11 (XLS 21 KB)—KEGG analysis of DEGs between the bile salt group and the control group

Supplementary file12 (XLS 4 KB)—KEGG analysis of DEGs between the bile salt-GTP and the bile salt group

Supplementary file13 (XLS 203 KB)—All genes in the control group, the bile salt group, and the bile salt-GTP

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Zhang, L., Xie, J., Zhang, Z. et al. RNA-Seq Transcriptomic Analysis of Green Tea Polyphenols Modulation of Differently Expressed Genes in Enterococcus faecalis Under Bile Salt Stress. Curr Microbiol 79, 147 (2022). https://doi.org/10.1007/s00284-022-02844-2

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  • DOI: https://doi.org/10.1007/s00284-022-02844-2

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