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Biosynthesis of Cyclic Lipopeptides by Bacillus velezensis Bs006 and its Antagonistic Activity are Modulated by the Temperature and Culture Media Conditions

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Abstract

Antagonistic activity of strains from Bacillus species has made them among the preferred agricultural biological control agents against phytopathogenic fungi. These microorganisms’ success is mostly based on the production of antagonistic secondary metabolites, mainly those of the non-ribosomal cyclic lipopeptides (CLPs) nature, which can affect phytopathogens directly (iturins and fengycins) or indirectly (surfactins and fengycins). However, abiotic factors in the target site can influence the behavior of the biocontrol traits, but to date, few studies attempting to decipher this kind of interaction have been conducted. This work aimed to evaluate the effect of temperature and culture medium on growth, antagonistic activity against Fusarium oxysporum f. sp. physali (Foph), and the profile of CLPs produced by Bacillus velezensis Bs006. The data showed that measured traits in Bs006 varied with temperature and medium interaction. The concentration of CLPs, as well as the antagonistic activity against Foph, was increased as the nutritional wealth, temperature, and time of incubation increased. The concentration of fengycins and iturins was higher than surfactins at high temperatures. However, a bacteriostatic effect was detected with a combination of Landy medium and 15 °C, which prevented both the biosynthesis of CLPs and the antagonistic activity. The results of this work highlight the importance of abiotic conditions of the target site where a biocontrol agent will be applied to stay active and develop its full antagonistic potential. This response by Bs006 could partly explain the variability of its biocontrol efficacy in the Foph-golden berry pathosystem.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The strains B. velezensis Bs006 and F. oxysporum f. sp. physali Map5 from AGROSAVIA Microorganisms Working Collection were evaluated in the research that gave rise to this paper. The authors acknowledge Laurent Franzil by technical support in the chromatography laboratory, and Drs. Felipe Borrero Echeverry and Andrea Paola Zuluaga by critical review of the manuscript.

Funding

C.A. Moreno-Velandia’s thesis was supported by commission studies for doctorate program from the Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA) and a grant from Programa Nacional de Formación de Investigadores, Becas Colciencias, Colombia, Doctorado Nacional 567. This research was funded by AGROSAVIA and Ministerio de Agricultura y Desarrollo Rural through TV15 and TV16 agreements for the development of project 687. Microbial Processes and Interactions Laboratory, at Gembloux Agro-Bio Tech, University of Liege also contributed for the development of this work.

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Authors and Affiliations

  1. Corporación Colombiana de Investigación Agropecuaria—AGROSAVIA, Km 14 vía Bogotá a Mosquera, 250047, Mosquera, Colombia

    Carlos A. Moreno-Velandia & Alba M. Cotes

  2. Microbial Processes and Interactions Laboratory, Terra Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2 5030, Gembloux, Belgium

    Marc Ongena

  3. Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, 36849, USA

    Joseph W. Kloepper

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  1. Carlos A. Moreno-Velandia
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CAM-V: conceptualization, methodology, investigation, formal analysis, and writing—original draft. MO: conceptualization, methodology, resources, and writing—review & editing. JWK: writing—review & editing. AMC: conceptualization, writing—review & editing, supervision, project administration, and funding acquisition.

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Correspondence to Carlos A. Moreno-Velandia.

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Moreno-Velandia, C.A., Ongena, M., Kloepper, J.W. et al. Biosynthesis of Cyclic Lipopeptides by Bacillus velezensis Bs006 and its Antagonistic Activity are Modulated by the Temperature and Culture Media Conditions. Curr Microbiol 78, 3505–3515 (2021). https://doi.org/10.1007/s00284-021-02612-8

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