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Uliginosibacterium aquaticum sp. nov., Isolated from a Freshwater Lake

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Abstract

A Gram-stain-negative, aerobic, chemoheterotrophic, rod-shaped bacterium motile by a polar flagellum, designated IMCC34675T, was isolated from Chungju Lake, an artificial freshwater reservoir in Republic of Korea. The 16S rRNA gene sequence analysis indicated that strain IMCC34675T belongs to the genus Uliginosibacterium, sharing ≤ 97.1% sequence similarities with the type strains of the genus. Whole genome sequencing of strain IMCC34675T revealed a 4.1 Mbp of genome size with 62.4% of the DNA G + C content. The IMCC34675T genome shared 73.3% of average nucleotide identity and 19.9% of digital DNA-DNA hybridization values to the genome of Uliginosibacterium gangwonense DSM 18521T, the type species of the genus. The major fatty acids of strain IMCC34675T were summed feature 3 (comprising C16:1ω6c and/or C16:1ω7c) and C16:0. The respiratory quinone detected in the strains was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, one aminolipid, and five unidentified lipids. Based on the phylogenetic and phenotypic characterization, strain IMCC34675T was considered to represent a novel species within the genus Uliginosibacterium, for which the name Uliginosibacterium aquaticum sp. nov. is proposed with IMCC34675T (= KACC 21758T = NBRC 114418T) as the type strain.

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Funding

This study was supported by the research grant “Survey of freshwater organisms and specimen collection (Prokaryotes)” from Nakdonggang National Institute of Biological Resources of the Ministry of Environment in Korea and by Inha University Research Grant.

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Author notes

  1. Jaeho Song and Mirae Kim are these authors contributed equally to this work.

Authors and Affiliations

  1. Microbial Research Division, Honam National Institute of Biological Resources, 99 Gohadoan-gil, Mokpo, Republic of Korea

    Jaeho Song

  2. Department of Biological Sciences and Bioengineering, Inha University, Incheon, 22212, Republic of Korea

    Mirae Kim, Miri S. Park, Yochan Joung, Ilnam Kang & Jang-Cheon Cho

Authors
  1. Jaeho Song
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  2. Mirae Kim
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  3. Miri S. Park
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  6. Jang-Cheon Cho
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Contributions

Conceptualization, JS, MK, and J-CC; Data curation, JS, MK, and J-CC; Funding acquisition, J-CC; Investigation, JS, YJ, MK, and IK; Resources, YJ; Supervision, J-CC; Visualization, JS, MK, and IK; Writing-original draft, JS; Writing-review &editing, JS, MSP, IK, and J-CC. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Jang-Cheon Cho.

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Song, J., Kim, M., Park, M.S. et al. Uliginosibacterium aquaticum sp. nov., Isolated from a Freshwater Lake. Curr Microbiol 78, 3381–3387 (2021). https://doi.org/10.1007/s00284-021-02605-7

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