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Differences in Soil Microbial Community Composition Between Suppressive and Root Rot-Conducive in Tobacco Fields

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Abstract

Soil microorganism has a profound influence on planting growth and disease suppression. However, the difference in microbial community structure between suppressive and root rot-conducive soil and the mechanism of controlling soil-borne diseases by microorganisms in suppressive soil were not clear. To provide a theoretical foundation for prevention and control of root rot, this paper investigated the change of community structure in rhizosphere soil between suppressive and root rot-conducive tobacco fields. Soil samples were collected during before transplanting, vigorous growing period, and mature period of the tobacco, and bacteria and fungi were analyzed using 16S rRNA and 18S rRNA gene sequencing, respectively. Results showed that bacteria were more sensitive to the change between suppressive and root rot-conducive soil, and fungi were more sensitive to the change of different tobacco growth periods. Compared with conducive soil, tobacco suppressive soil can resist the invasion of pathogens, especially fungi, by regulating soil microbial community structure, and the potential pathogen Boeremia was always lower. Fusarium, the root rot pathogen, decreased rapidly in the mature period in suppressive soil. Moreover, norank_o_Gaiellales and unclassified_f_Trichocomaceae had a critical role in suppressive soil in the process of inhibiting root rot, which was obvious in the mature stage. Overall, the results indicated that the composition and structure of the microbial community significantly altered between suppressive and conducive soil along with the growth of tobacco, and suppressive soil could inhibit the occurrence of soil-borne diseases by boosting beneficial bacteria and inhibiting the potential pathogens.

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Acknowledgements

The support of this study from the Scientific and Technological Project of Sichuan Tobacco Company Liangshan Prefecture, China (Grant Number: LSYC201803).

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Authors and Affiliations

  1. College of Ecology and Environment, Zhengzhou University, Zhengzhou, China

    Yaru Ding, Hongli Li & Yan Wang

  2. Liangshan Prefecture Company, Tobacco Company of Sichuan, Xichang, China

    Yulan Chen

  3. Ningnan Branch, Liangshan Prefecture Company, Tobacco Company of Sichuan, Ningnan, Liangshan, Sichuan, China

    Zhengquan Lin

  4. Dechang Branch, Liangshan Prefecture Company, Tobacco Company of Sichuan, Dechang, Liangshan, Sichuan, China

    Yangyang Tuo

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  1. Yaru Ding
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  2. Yulan Chen
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  5. Hongli Li
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  6. Yan Wang
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Contributions

HL, YW designed this experiment. YC, ZL and YT executed the experiment. YD finished the manuscript.

Corresponding author

Correspondence to Hongli Li.

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Conflict of interest

Author Yulan Chen was employed by Liangshan Prefecture Company, Tobacco Company of Sichuan and Zhengquan Lin was employed by Ningnan Branch, Liangshan Prefecture Company, Tobacco Company of Sichuan, and Yangyang Tuo was employed by Dechang Branch, Liangshan Prefecture Company, Tobacco Company of Sichuan. The remaining authors state that this work was conducted without any potential economic and conflict of interest.

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Ding, Y., Chen, Y., Lin, Z. et al. Differences in Soil Microbial Community Composition Between Suppressive and Root Rot-Conducive in Tobacco Fields. Curr Microbiol 78, 624–633 (2021). https://doi.org/10.1007/s00284-020-02318-3

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  • DOI: https://doi.org/10.1007/s00284-020-02318-3

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