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Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila

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Abstract

Phage therapy is an alternative approach to overcome the problem of multidrug-resistant bacteria. Here, a novel bacteriophage AhyVDH1, which infects Aeromonas hydrophila 4572, was isolated and its morphology, one-step growth curve, lytic activity, stability under various conditions, and genome were investigated. Transmission electron microscopy revealed that AhyVDH1 has an icosahedral head 49 nm in diameter and a contractile tail 127 nm in length, suggesting that it belongs to the family Myoviridae. AhyVDH1 showed strong adsorption to the surface of A. hydrophila 4572 (90% in 10 min). The latent period of AhyVDH1 was shown to be 50 min, and the burst size was 274 plaque-forming unit/infected cell. AhyVDH1 was stable at 30 °C for 1 h and lost infectivity after20 min of heating at 60 °C. Infectivity remained unaffected at pH 6–7 for 1 h, while the bacteriophage was inactivated at pH < 4 or > 11. AhyVDH1 has a 39,175-bp genome, with a 58% G + C content and 59 open reading frames. BLAST analysis indicated that the genome sequence of phage AhyVDH1 was related to that of Aeromonas phage Ahp2. Both time and MOI-dependent in vitro A. hydrophila growth inhibition were observed with AhyVDH1.Re-growth of the host bacteria appeared about 12 h after treatment, suggesting its potential therapeutic value in treating A. hydrophila infections, but phage cocktails should be developed.

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Funding

This funding was supported by National Natural Science Foundation of China [Grant Nos. 31660042, 31660001, 31660089, 32071570] and Yunnan Provincial Science and Technology Department [Grant No. 2018IA100].

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Authors and Affiliations

  1. Yunnan Engineering Laboratory of Soil Fertility and Pollution Remediation, Yunnan Agricultural University, Kunming, 650201, People’s Republic of China

    Yahui Cheng, Dongye Gao, Yunsheng Xia, Ziyi Wang & Shiying Zhang

  2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Meng Bai, Xiaolong Cui, Yongxia Wang & Wei Xiao

  3. Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, 650500, People’s Republic of China

    Kaijun Luo, Xiaolong Cui, Yongxia Wang & Wei Xiao

  4. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, People’s Republic of China

    Xiaolong Cui

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  1. Yahui Cheng
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Cheng, Y., Gao, D., Xia, Y. et al. Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila. Curr Microbiol 78, 329–337 (2021). https://doi.org/10.1007/s00284-020-02279-7

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