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Characterization of Bacterial Communities Associated with Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae) and its Host Phoenix sylvestris

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Abstract

This study measured the changes of microorganisms in the midgut and habitat niche of Rhynchophorus ferrugineus Olivier, an invasive quarantine pest, by Illumina sequencing. The bacterial diversity in the R. ferrugineus larvae midgut and their habitat niche was compared to the uninfected P. sylvestris. The Proteobacteria and Firmicutes occupied a dominant position in the R. ferrugineus midgut and infected P. sylvestris, while in the uninfected P. sylvestris the predominant bacterial phylum was the Cyanobacteria. Enterobacter, Dysgonomonas, and Entomoplasma were the dominant bacterial genera in R. ferrugineus midgut and also within the infected trees and uninfected trees with low relative abundance. These bacteria could be exploited as the biopesticide vector to control R. ferrugineus population. Besides, Sphingobacterium, Shinella, and Rhodobacter genera had the same distribution pattern in the infected and uninfected P. sylvestris, and these bacteria were not found in the midgut of R. ferrugineus. Interestingly, Paludibacter and Parabacteroides were only distributed in the wood fiber of the infected P. sylvestris, which could be used as potential microbial markers to detect if the palm plants are damaged by the R. ferrugineus. The results of this study will be beneficial to the development of control strategies for R. ferrugineus.

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Acknowledgements

This research was funded by National Key Research and Development Program of China, Grant Number 2017YFD0600105; National Natural Science Foundation of China, Grant Number U1905201; National Natural Science Foundation of China, grant number 31601905; Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry University, Grant Number xjq201614; China Postdoctoral Science Foundation, Grant Number 2017M612107; Science and Technology Program of Fujian Province, Grant Number 2018N5002; Forestry Science Research Project of Fujian Forestry Department, Grant Number Minlinke (2017) 03; Forest Science Peak Project of College of Forestry, Fujian Agriculture and Forestry University, Grant Numbers 71201800720, 71201800753, 71201800779; Undergraduate Training Program for Innovation and Entrepreneurship of China, Grant Number 201910389009.

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Authors and Affiliations

  1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350000, China

    Qiliao Liao, Yajie Guo, Jianshuang Zhou, Yi Wan, Liangjing Sheng, Feiping Zhang, Songqing Wu & Shuangquan Zou

  2. Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry University, Fuzhou, 350000, China

    Yajie Guo, Jianshuang Zhou, Yi Wan, Liangjing Sheng, Feiping Zhang & Songqing Wu

  3. Department of Microbiology & Molecular Genetics, University of California, Irvine, CA, 92697-4025, USA

    Rebeca Carballar-Lejarazú

  4. Fujian Colleges and Universities Engineering Research Institute of Conservation and Utilization of Natural Bioresources, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350000, China

    Shuangquan Zou

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  1. Qiliao Liao
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Contributions

SQZ, SQW and FPZ contributed to the conception and design of the study; QLL, JSZ and YW obtained the metagenome samples; QLL, YJG, and LJS performed the data analysis; QLL, YJG, SQW, YW, and RCL drafted the manuscript; and QLL, SQW, and YJG reviewed the manuscript. All authors read and approved the submitted version.

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Correspondence to Songqing Wu or Shuangquan Zou.

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Liao, Q., Guo, Y., Zhou, J. et al. Characterization of Bacterial Communities Associated with Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae) and its Host Phoenix sylvestris. Curr Microbiol 77, 3321–3329 (2020). https://doi.org/10.1007/s00284-020-02196-9

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