Abstract
Non-natural 2-methyl-1-butanol (2 MB) has been biosynthesized through the modification of metabolic pathways using Corynebacterium crenatum, a non-model host. However, its production capacity is not effectively improved. In this study, the fermentation process was strengthened through factor combination design (FCD) for enhancing the production of 2 MB. Our results showed that the highest production of 2 MB, 3-methyl-1-butanol (3 MB), ethanol, and total solvent was 4.87 ± 0.39 g/L, 3.57 ± 0.21 g/L, 5.74 ± 0.43 g/L, and 14.18 g/L, respectively, under the optimal fermentation conditions. The optimal fermentation conditions were determined through the FCD to be as follows: pH of 6.5, IPTG concentration of 1.2 mM, fermentation temperature of 32 °C, and fermentation time of 96 h. This study provides a significant guidance for the optimal control technology of the genetically engineered C. crenatum, and also a useful reference for the industrial production of 2 MB via the microbial fermentation approach.
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Acknowledgements
This work was supported by the Open Project Program of the State Key Laboratory of Petroleum Pollution Control (Grant Nos. PPC2017004), the CNPC Research Institute of Safety and Environmental Technology, and National Natural Science Foundation and frontier research program of Chongqing (Grant Nos. cstc2017jcyjA02138), Chongqing Science Committee, Key laboratory of Degraded and Unused Land Consolidation Engineering, the Ministry of Natural and Resources (Grant No. SXDJ2019).
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Haifeng Su. participated in the conception, design, data collection and analysis, and drafted the manuscript. JiaFu Lin assisted the laboratory work, results interpretation and the manuscript revision. Hua Chen participated in the partial discussion of the results and revised the manuscript. All authors read and approved the final manuscript.
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Su, H., Chen, H. & Lin, J. Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum. Curr Microbiol 77, 1699–1706 (2020). https://doi.org/10.1007/s00284-020-01961-0
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DOI: https://doi.org/10.1007/s00284-020-01961-0