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Streptomyces songpinggouensis sp. nov., a Novel Actinomycete Isolated from Soil in Sichuan, China

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Abstract

During a screening for novel and biotechnologically useful actinobacteria, a novel actinobacteria with weak antifungal activity, designated strain NEAU-Spg19T, was isolated from a soil sample collected from pine forest in Songpinggou, Sichuan, southwest China. The strain was characterized using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth occurred at a temperature range of 10–30 °C, pH 5.0–11.0 and NaCl concentrations of 0–5 %. The cell wall peptidoglycan consisted of LL-diaminopimelic acid and glycine. The major menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The phospholipid profile contained diphosphatidylglycerol (DPG), phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were iso-C15:0, iso-C16:0, and C16:0. 16S rRNA gene sequence similarity studies showed that strain NEAU-Spg19T belongs to the genus Streptomyces with the highest sequence similarities to Streptomyces tauricus JCM 4837T (98.6 %) and Streptomyces rectiviolaceus JCM 9092T (98.3 %). Some physiological and biochemical properties and low DNA–DNA relatedness values enabled the strain to be differentiated from S. tauricus JCM 4837T and S. rectiviolaceus JCM 9092T. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NEAU-Spg19T represents a novel species of the genus Streptomyces, for which the name Streptomyces songpinggouensis sp. nov. is proposed. The type strain is NEAU-Spg19T (=CGMCC 4.7140T=DSM 42141T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Technology R&D Program (No. 2012BAD19B06), the National Natural Science Foundation of China (Nos. 31471832, 31171913, 31500010, 31572070 and 31372006) and Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

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    Authors and Affiliations

    1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Xuejiao Guan, Wenchao Li, Chongxi Liu, Pinjiao Jin, Siyu Guo, Xiangjing Wang & Wensheng Xiang

    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

      Wensheng Xiang

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    1. Xuejiao Guan
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    Correspondence to Xiangjing Wang or Wensheng Xiang.

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    Xuejiao Guan and Wenchao Li have contributed equally to this work.

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    Guan, X., Li, W., Liu, C. et al. Streptomyces songpinggouensis sp. nov., a Novel Actinomycete Isolated from Soil in Sichuan, China. Curr Microbiol 73, 796–801 (2016). https://doi.org/10.1007/s00284-016-1128-3

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