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Gibberella moniliformis AH13 with Antitumor Activity, an Endophytic Fungus Strain Producing Triolein Isolated from Adlay (Coix lacryma-jobi: Poaceae)

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Abstract

In this study, the isolation of an endophytic fungus from the leaves of the medicinal herb adlay (Coix lacryma-jobi L. var. ma-yuen Stapf) is reported for the first time. The fungus produced Triolein (trioleoylglycerol), a major constituent of triacylglycerols (TAGs) of adlay, in rice medium under shake-flask and bench-scale fermentation conditions. The fungus was identified as Gibberella moniliformis (Fusarium verticillioides) by its morphology and authenticated by ITS analysis (ITS1 and ITS2 regions and the intervening 5.8S rDNA region). Triolein was identified by HPLC-ELSD coupled with APCI-MS and confirmed through comparison with authentic standard. The concentration of triolein produced by G. moniliformis AH13 reached 2.536 ± 0.006 mg/g dry weight of mycelium. Moreover, the EtOAc extract of G. moniliformis AH13 showed strong antitumor activity against four types of tumor cells (A549, HCT116, MDA-MB-231, and SW1990). These results suggest that G. moniliformis AH13 in adlay has significant scientific and industrial potential to meet the pharmaceutical demands and sustainable energy requirements for TAGs in a cost-effective, easily accessible, and reproducible way and is also a potential novel source of natural antitumor bioactive agents.

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, 200433, People’s Republic of China

    Min Jia, Qian-Liang Ming, Qiao-Yan Zhang, Yu Chen, Nuo Cheng, Wen-wen Wu, Ting Han & Lu-Ping Qin

  2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, 325 Guohe Road, Shanghai, 200433, People’s Republic of China

    Lu-Ping Qin

Authors
  1. Min Jia
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  2. Qian-Liang Ming
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  3. Qiao-Yan Zhang
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  4. Yu Chen
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  5. Nuo Cheng
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  6. Wen-wen Wu
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  7. Ting Han
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  8. Lu-Ping Qin
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Corresponding authors

Correspondence to Ting Han or Lu-Ping Qin.

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Min Jia and Qian-Liang Ming contributed equally to this paper

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Jia, M., Ming, QL., Zhang, QY. et al. Gibberella moniliformis AH13 with Antitumor Activity, an Endophytic Fungus Strain Producing Triolein Isolated from Adlay (Coix lacryma-jobi: Poaceae). Curr Microbiol 69, 381–387 (2014). https://doi.org/10.1007/s00284-014-0590-z

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  • DOI: https://doi.org/10.1007/s00284-014-0590-z

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