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Isolation and Identification of Environmental Mycobacteria in the Waters of a Hemodialysis Center

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Abstract

The use of poorly treated water during hemodialysis may lead to contamination with nontuberculous mycobacteria (NTM). This study aimed to isolate and identify NTM species in the water of a Brazilian hemodialysis center. We collected 210 samples of water from the hydric system of the unit (post-osmosis system, hemodialysis rooms, reuse system, and hemodialysis equipment) and from the municipal supply network; we isolated the NTM by a classic microbiological technique and identified them by the PCR restriction enzyme pattern of the hsp65 gene (PRA). Fifty-one (24.3 %) of the collected samples tested positive for NTM; both the municipal supply network (2 samples, 3.2 %) and the hydric system of the hemodialysis center (49 samples, 96.1 %) contained NTM. We isolated and identified potentially pathogenic bacteria such as Mycobacterium lentiflavum (59.0 %) and M. kansasii (5.0 %), as well as rarely pathogenic bacteria like M. gordonae (24.0 %), M. gastri (8.0 %), and M. szulgai (4.0 %). The ability of NTM to cause diseases is well documented in the literature. Therefore, the identification of NTM in the water of a Brazilian hemodialysis center calls for more effective water disinfection procedures in this unit.

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Authors and Affiliations

  1. Laboratory of Research in Applied Microbiology, University of Franca, Franca, 14404-600, Brazil

    Flávio Garcia Sartori, Luís Fernando Leandro, Lilian Bueno Montanari, Maria Gorete Mendes de Souza, Regina Helena Pires & Carlos Henrique Gomes Martins

  2. Laboratory of Mycobacteria, Adolfo Lutz Institute, Ribeirão Preto, 14085-410, Brazil

    Daisy Nakamura Sato

  3. Department of Biological Sciences, State University of São Paulo, Araraquara, 14801-360, Brazil

    Clarice Queico Fujimura Leite & Karina de Andrade Prince

Authors
  1. Flávio Garcia Sartori
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  2. Luís Fernando Leandro
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  3. Lilian Bueno Montanari
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  4. Maria Gorete Mendes de Souza
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  5. Regina Helena Pires
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  6. Daisy Nakamura Sato
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  7. Clarice Queico Fujimura Leite
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  8. Karina de Andrade Prince
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  9. Carlos Henrique Gomes Martins
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Correspondence to Carlos Henrique Gomes Martins.

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Sartori, F.G., Leandro, L.F., Montanari, L.B. et al. Isolation and Identification of Environmental Mycobacteria in the Waters of a Hemodialysis Center. Curr Microbiol 67, 107–111 (2013). https://doi.org/10.1007/s00284-013-0341-6

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  • DOI: https://doi.org/10.1007/s00284-013-0341-6

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